Detailed information of TA system TA025654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1914281..1914864
• Replicon: chromosome
• Accession: NC_016027
• Organism: Gluconacetobacter medellinensis NBRC 3288

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GLX_RS17260
• Protein ID: WP_081477885.1
• Coordinates: 1914281..1914625 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G2I7M1
• Locus tag: GLX_RS08955
• Protein ID: WP_014105651.1
• Coordinates: 1914622..1914864 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLX_RS08945	1910212..1910967	+	756	Protein_1770	integrase	-
GLX_RS08950	1911100..1912179	-	1080	WP_014104060.1	IS5 family transposase	-
GLX_RS17255	1912381..1914051	+	1671	WP_014105650.1	AAA family ATPase	-
GLX_RS17260	1914281..1914625	-	345	WP_081477885.1	endoribonuclease MazF	Toxin
GLX_RS08955	1914622..1914864	-	243	WP_014105651.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GLX_RS08960	1915181..1916467	+	1287	WP_014105652.1	integrase	-
GLX_RS08965	1916558..1917637	-	1080	WP_014105653.1	IS5 family transposase	-
GLX_RS18460	1917722..1918087	+	366	WP_158309230.1	hypothetical protein	-
GLX_RS08970	1918483..1919409	-	927	WP_014105654.1	Bpu10I family restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1911100..1932917	21817

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (13-112)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12546.75 Da
• Isoelectric Point: 10.1798

>T25654 WP_081477885.1 NC_016027:c1914625-1914281 [Komagataeibacter medellinensis NBRC 3288]
VSAKVKTAWVPDCGDIVWLEFDPQAGREQAGHRPAVVLSPASYNAKAGMMLCCPTTTKIKGYPFEVALTGKTASVVLSDQ
LRSLDWRVRKAKLKGKVLPEELEAIRQRIRLLLG

Nucleotide

• Length: 345 bp

>T25654 NC_016027:c1914625-1914281 [Komagataeibacter medellinensis NBRC 3288]
GTGAGTGCCAAAGTAAAGACTGCTTGGGTTCCTGATTGCGGGGACATCGTTTGGCTGGAATTTGATCCGCAGGCGGGCAG
GGAGCAGGCAGGACACCGGCCTGCTGTTGTGTTGAGCCCCGCCAGTTATAACGCTAAAGCTGGAATGATGCTGTGCTGTC
CGACAACGACCAAAATCAAAGGCTACCCCTTTGAAGTGGCTCTGACTGGAAAAACTGCCAGTGTGGTTTTGTCAGATCAG
CTCAGAAGTCTGGATTGGCGCGTGAGAAAGGCAAAGCTCAAAGGCAAAGTTTTACCAGAAGAGCTTGAGGCCATCCGGCA
GCGTATCAGATTGCTGCTTGGGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8680.90 Da
• Isoelectric Point: 4.4488

>AT25654 WP_014105651.1 NC_016027:c1914864-1914622 [Komagataeibacter medellinensis NBRC 3288]
MQGVVRKWGNSAAIRLPAGVLEAVSLKIDQAVDVREEDGRIIIEPVKAVPLKLEELVSGITAENRHDEMDFGPSVGGESW

Nucleotide

• Length: 243 bp

>AT25654 NC_016027:c1914864-1914622 [Komagataeibacter medellinensis NBRC 3288]
ATGCAAGGCGTTGTCAGGAAATGGGGAAACAGTGCAGCCATTCGTCTTCCCGCTGGTGTGCTTGAAGCCGTCAGTCTGAA
GATCGATCAGGCCGTCGATGTGCGGGAAGAAGATGGACGGATTATCATTGAGCCAGTCAAAGCAGTTCCTCTCAAACTGG
AAGAATTAGTGTCAGGTATTACGGCCGAGAACCGCCATGATGAAATGGACTTCGGTCCATCTGTTGGTGGTGAAAGCTGG
TGA

Structures

Antitoxin

• AlphaFold DB: G2I7M1