Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4216353..4217110 Replicon chromosome
Accession NZ_CP103751
Organism Enterobacter asburiae strain 2497

Toxin (Protein)


Gene name tacT Uniprot ID A0A7H0RWY0
Locus tag M5T12_RS20830 Protein ID WP_007372585.1
Coordinates 4216353..4216835 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A1D8JMH3
Locus tag M5T12_RS20835 Protein ID WP_007372584.1
Coordinates 4216826..4217110 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T12_RS20810 (M5T12_20810) 4211413..4212693 - 1281 WP_071532028.1 type 4b pilus protein PilO2 -
M5T12_RS20815 (M5T12_20815) 4212696..4214369 - 1674 WP_007372588.1 PilN family type IVB pilus formation outer membrane protein -
M5T12_RS20820 (M5T12_20820) 4214389..4214832 - 444 WP_007372587.1 type IV pilus biogenesis protein PilM -
M5T12_RS20825 (M5T12_20825) 4214834..4216120 - 1287 WP_007372586.1 TcpQ domain-containing protein -
M5T12_RS20830 (M5T12_20830) 4216353..4216835 - 483 WP_007372585.1 GNAT family N-acetyltransferase Toxin
M5T12_RS20835 (M5T12_20835) 4216826..4217110 - 285 WP_007372584.1 DUF1778 domain-containing protein Antitoxin
M5T12_RS20840 (M5T12_20840) 4217266..4217766 - 501 WP_007372583.1 single-stranded DNA-binding protein -
M5T12_RS20845 (M5T12_20845) 4217842..4218333 - 492 WP_007372582.1 STY4534 family ICE replication protein -
M5T12_RS20850 (M5T12_20850) 4218966..4220978 - 2013 WP_007372580.1 DNA topoisomerase III -
M5T12_RS20855 (M5T12_20855) 4220996..4221718 - 723 WP_007372579.1 TIGR03761 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4123424..4321175 197751


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-146)

Antitoxin

Vibrio phage ICP1, Orf50

(1-93)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17756.41 Da        Isoelectric Point: 7.3823

>T256520 WP_007372585.1 NZ_CP103751:c4216835-4216353 [Enterobacter asburiae]
MEINVTAPALLTEEHVLHPFDCGNAVLDDWLRRRALKNQHLNASRTFVICPEGTARVVGYYSLATGSVSHVDLSRSLRQN
MPDPVPVVLLGRLAVDVSTQGNNFGKWLLNDAVMRVSNLADQVGIKAIMVHAIDERAKAFYEYFGFVQSPVAANTLFYKI

Download         Length: 483 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 11078.63 Da        Isoelectric Point: 8.5684

>AT256520 WP_007372584.1 NZ_CP103751:c4217110-4216826 [Enterobacter asburiae]
MQTPVRKSVRDKQINIRATDEERAVIDYAASLVNKNRTDFIIERAVHEAQNIILDQRVFVLDDARYQAFIRQLEAPVQNV
EGRQRLMDVKPEWK

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H0RWY0

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JMH3

References