Detailed information of TA system TA025652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 213166..214686
• Replicon: chromosome
• Accession: NC_016026
• Organism: Micavibrio aeruginosavorus ARL-13

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: G2KP39
• Locus tag: MICA_RS00940
• Protein ID: WP_014101770.1
• Coordinates: 213166..214455 (-)
• Length: 430 a.a.

Antitoxin (Protein)

• Gene name: HipB2
• Uniprot ID: -
• Locus tag: MICA_RS00945
• Protein ID: WP_014101771.1
• Coordinates: 214462..214686 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MICA_RS00930	210012..211316	-	1305	WP_014101768.1	M50 family metallopeptidase	-
MICA_RS00935	211329..213008	-	1680	WP_014101769.1	energy-dependent translational throttle protein EttA	-
MICA_RS00940	213166..214455	-	1290	WP_014101770.1	type II toxin-antitoxin system HipA family toxin	Toxin
MICA_RS00945	214462..214686	-	225	WP_014101771.1	helix-turn-helix transcriptional regulator	Antitoxin
MICA_RS00950	214911..215645	+	735	WP_014101772.1	hypothetical protein	-
MICA_RS00955	215642..216433	+	792	WP_014101773.1	TerD family protein	-
MICA_RS00960	216472..216978	-	507	WP_014101774.1	hypothetical protein	-
MICA_RS00965	217062..218510	-	1449	WP_014101775.1	exodeoxyribonuclease VII large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 430 a.a.
• Molecular weight: 47458.18 Da
• Isoelectric Point: 6.7313

>T25652 WP_014101770.1 NC_016026:c214455-213166 [Micavibrio aeruginosavorus ARL-13]
MQATPLEKLRVYLRGEYVGALTSDSGTLSFAYDGAYCEKKDAPKLSVSLPIQKETFSHHAAAPFFSGLLPDGDVRKHLAK
HLKLSPENIFGLLEAVGGDCAGAVSLYKEHDLLNNTTNPTYKVLSDTEAAEILSSLNHRPFLVGEEGIRMSGAGAQDKLM
VSFVGKKIALPTGQTPSTHIIKPPIKGAENSVQNEFFCMRLAQKIGLPAPDTKILYVDQNPYYCVTRYDRETNETGEVAR
LHQEDFCQALHIPPELKYENDSGPSLEACFNLLDQRIRSGSMPGKDKLTFLYGVIFNVLIGNGDAHAKNFSILYRNGAET
LAPFYDLLCTEIYNKNGKFRMAMKIDGHYMPHTMSARNWKNFAASIGIAPSLMHKALRKVINDVSREATGLYLDLHSDTG
TTSPVYEKIMGVIASNTQELELQLLTWDT

Nucleotide

• Length: 1290 bp

>T25652 NC_016026:c214455-213166 [Micavibrio aeruginosavorus ARL-13]
ATGCAAGCAACACCTCTCGAAAAATTGCGCGTCTATTTACGCGGAGAATACGTTGGGGCACTCACGTCTGATTCCGGCAC
ATTGTCTTTCGCATACGATGGGGCTTATTGCGAAAAAAAAGATGCCCCGAAATTATCAGTATCTTTACCCATACAAAAAG
AAACATTTTCCCACCATGCAGCAGCCCCCTTCTTCTCGGGACTTTTACCTGATGGAGACGTACGTAAGCATTTAGCAAAA
CACTTAAAACTATCCCCTGAAAATATATTTGGTCTTCTGGAAGCCGTTGGCGGCGACTGCGCTGGCGCAGTCTCATTGTA
CAAAGAGCATGACCTCCTAAACAATACAACCAACCCAACCTACAAAGTCCTGAGCGATACTGAGGCGGCAGAGATTTTAT
CGTCGCTCAATCATCGTCCATTCCTTGTCGGTGAGGAAGGTATCCGTATGTCTGGCGCTGGCGCACAAGACAAACTTATG
GTTTCTTTTGTTGGTAAAAAAATAGCCCTTCCAACAGGACAAACCCCATCAACGCACATAATAAAGCCTCCAATAAAAGG
CGCTGAAAATTCTGTGCAAAATGAATTTTTTTGCATGCGGCTTGCGCAAAAAATTGGTTTACCTGCACCTGACACAAAAA
TTCTATATGTAGATCAAAACCCCTATTACTGCGTAACTCGTTATGATCGGGAAACAAACGAAACGGGGGAAGTCGCACGA
CTGCATCAGGAAGATTTTTGCCAGGCTTTACACATTCCCCCAGAATTAAAATATGAGAACGATAGCGGCCCCAGCCTTGA
AGCATGCTTCAATCTTTTAGATCAACGAATAAGATCAGGAAGCATGCCCGGAAAAGACAAGCTCACATTCCTATATGGTG
TTATTTTTAACGTTCTTATCGGTAATGGAGATGCACACGCAAAGAATTTTTCCATTCTTTACAGAAATGGCGCCGAAACC
CTCGCGCCTTTTTATGATTTGCTCTGCACAGAAATTTACAATAAGAACGGAAAATTCAGAATGGCTATGAAAATCGATGG
TCATTACATGCCTCACACCATGTCTGCCCGTAACTGGAAAAATTTCGCAGCATCTATCGGTATTGCGCCATCACTCATGC
ACAAAGCACTGCGCAAAGTCATAAATGACGTATCAAGAGAGGCAACTGGGCTCTACCTCGACCTCCATTCAGATACCGGT
ACGACCTCGCCGGTATATGAAAAAATTATGGGTGTTATCGCCTCAAACACACAAGAACTTGAGCTCCAGCTACTAACATG
GGACACCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8198.65 Da
• Isoelectric Point: 9.8143

>AT25652 WP_014101771.1 NC_016026:c214686-214462 [Micavibrio aeruginosavorus ARL-13]
MEQIIPNPKELGAIVREARKEQHLTQEELAGLTGTGRRFISELEQGKTTLELGKVLLVLAALGIALYALSKWKK

Nucleotide

• Length: 225 bp

>AT25652 NC_016026:c214686-214462 [Micavibrio aeruginosavorus ARL-13]
ATGGAGCAAATCATACCAAACCCAAAAGAGCTGGGCGCAATTGTACGAGAGGCACGCAAGGAACAGCATTTAACCCAAGA
AGAGCTGGCCGGACTAACCGGAACAGGACGGCGCTTTATCAGCGAACTGGAACAAGGCAAAACAACACTTGAACTTGGAA
AGGTTCTACTGGTGCTTGCCGCACTAGGCATCGCCCTGTACGCACTGAGCAAGTGGAAAAAATAA

Structures

Toxin

• AlphaFold DB: G2KP39