Detailed information of TA system TA025651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 231419..231815
• Replicon: chromosome
• Accession: NC_016025
• Organism: Candidatus Chloracidobacterium thermophilum B

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CABTHER_RS16740
• Protein ID: WP_081464913.1
• Coordinates: 231419..231568 (-)
• Length: 50 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G2LK18
• Locus tag: CABTHER_RS12015
• Protein ID: WP_014100923.1
• Coordinates: 231573..231815 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CABTHER_RS11995	226450..227034	-	585	WP_014100919.1	aminodeoxychorismate/anthranilate synthase component II	-
CABTHER_RS12000	227060..228547	-	1488	WP_014100920.1	anthranilate synthase component I family protein	-
CABTHER_RS12005	228544..229374	-	831	WP_041569955.1	bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase	-
CABTHER_RS12010	229597..230670	-	1074	WP_014100922.1	lipopolysaccharide heptosyltransferase II	-
CABTHER_RS16730	230937..231161	-	225	WP_081464911.1	type II toxin-antitoxin system HicA family toxin	-
CABTHER_RS16735	231158..231370	-	213	WP_081464912.1	type II toxin-antitoxin system HicB family antitoxin	-
CABTHER_RS16740	231419..231568	-	150	WP_081464913.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CABTHER_RS12015	231573..231815	-	243	WP_014100923.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CABTHER_RS12020	231928..233853	-	1926	WP_148264090.1	CocE/NonD family hydrolase	-
CABTHER_RS12025	233929..234450	-	522	WP_014100925.1	adenine phosphoribosyltransferase	-
CABTHER_RS12030	234717..234995	-	279	WP_014100926.1	acylphosphatase	-
CABTHER_RS12040	235264..235740	-	477	WP_014100927.1	DUF4157 domain-containing protein	-
CABTHER_RS12045	235759..236295	-	537	WP_014100928.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-49)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5379.32 Da
• Isoelectric Point: 7.8609

>T25651 WP_081464913.1 NC_016025:c231568-231419 [Chloracidobacterium thermophilum B]
MAYIPDSGDIVWIMCTPQAGHEQTRHRPALVLSPKSYNSKVGLALLCPI

Nucleotide

• Length: 150 bp

>T25651 NC_016025:c231568-231419 [Chloracidobacterium thermophilum B]
ATGGCATACATTCCCGACAGTGGTGACATCGTTTGGATAATGTGCACCCCACAAGCGGGGCATGAGCAGACACGGCATAG
ACCAGCTTTGGTTTTATCGCCGAAGAGTTACAACAGCAAAGTTGGTCTTGCCCTGCTCTGCCCAATATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8626.91 Da
• Isoelectric Point: 4.2508

>AT25651 WP_014100923.1 NC_016025:c231815-231573 [Chloracidobacterium thermophilum B]
MLAKIQKWGNSFALRIPKVFAAEAQLQNDSLVEISIVEGKIIITPAPAPGWTLDALLAGITEDNLHPEVETGFAVGNEAW

Nucleotide

• Length: 243 bp

>AT25651 NC_016025:c231815-231573 [Chloracidobacterium thermophilum B]
ATGCTCGCAAAAATTCAGAAATGGGGTAACAGTTTTGCCCTGCGAATACCGAAAGTCTTTGCTGCTGAAGCTCAGCTTCA
AAATGACTCACTTGTAGAAATCAGTATCGTCGAAGGCAAGATTATTATTACTCCGGCTCCAGCGCCAGGTTGGACACTTG
ACGCATTGCTGGCAGGTATCACCGAGGACAATCTTCATCCCGAAGTCGAGACGGGTTTTGCCGTAGGAAATGAAGCCTGG
TGA

Structures

Antitoxin

• AlphaFold DB: G2LK18