Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 111959..112602 Replicon plasmid pMB2791_1
Accession NZ_CP103746
Organism Escherichia coli strain 3090

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag M5T02_RS24300 Protein ID WP_001044768.1
Coordinates 112186..112602 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag M5T02_RS24295 Protein ID WP_001261287.1
Coordinates 111959..112189 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T02_RS24275 (107139) 107139..108272 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
M5T02_RS24280 (108535) 108535..110172 - 1638 WP_223201785.1 hypothetical protein -
M5T02_RS24285 (110223) 110223..111053 - 831 WP_223201786.1 hypothetical protein -
M5T02_RS24290 (111105) 111105..111653 - 549 WP_039002808.1 hypothetical protein -
M5T02_RS24295 (111959) 111959..112189 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M5T02_RS24300 (112186) 112186..112602 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
M5T02_RS24305 (112764) 112764..114902 - 2139 WP_039002805.1 AAA family ATPase -
M5T02_RS24310 (115367) 115367..116362 + 996 WP_000246636.1 hypothetical protein -
M5T02_RS24315 (116405) 116405..117298 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 117435..117938 503
- inside Conjugative plasmid ant(3'')-Ia / lnu(F) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / tet(M) / aac(3)-IId - 1..120324 120324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T256501 WP_001044768.1 NZ_CP103746:112186-112602 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT256501 WP_001261287.1 NZ_CP103746:111959-112189 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References