Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4194095..4194671 Replicon chromosome
Accession NZ_CP103745
Organism Escherichia coli strain 3090

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag M5T02_RS20285 Protein ID WP_044071852.1
Coordinates 4194384..4194671 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag M5T02_RS20280 Protein ID WP_000063160.1
Coordinates 4194095..4194397 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T02_RS20265 (4190691) 4190691..4192841 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5T02_RS20270 (4192891) 4192891..4193094 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5T02_RS20275 (4193105) 4193105..4194061 + 957 WP_001297640.1 GTPase -
M5T02_RS20280 (4194095) 4194095..4194397 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
M5T02_RS20285 (4194384) 4194384..4194671 - 288 WP_044071852.1 BrnT family toxin Toxin
M5T02_RS20290 (4194870) 4194870..4195566 - 697 Protein_3968 IS1 family transposase -
M5T02_RS20295 (4195881) 4195881..4197113 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
M5T02_RS20300 (4197154) 4197154..4198434 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4194870..4195079 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11296.72 Da        Isoelectric Point: 6.8821

>T256490 WP_044071852.1 NZ_CP103745:c4194671-4194384 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGNRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT256490 WP_000063160.1 NZ_CP103745:c4194397-4194095 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References