Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 1630433..1631066 Replicon chromosome
Accession NC_016023
Organism Bacillus coagulans 36D1

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag BCOA_RS24920 Protein ID WP_013860680.1
Coordinates 1630716..1631066 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag BCOA_RS24915 Protein ID WP_014096808.1
Coordinates 1630433..1630711 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCOA_RS24895 1626540..1627139 - 600 WP_014096804.1 rhomboid family intramembrane serine protease -
BCOA_RS24900 1627420..1627770 + 351 WP_014096805.1 holo-ACP synthase -
BCOA_RS24905 1627911..1628927 + 1017 WP_014096806.1 outer membrane lipoprotein carrier protein LolA -
BCOA_RS24910 1629129..1630280 + 1152 WP_014096807.1 alanine racemase -
BCOA_RS24915 1630433..1630711 + 279 WP_014096808.1 hypothetical protein Antitoxin
BCOA_RS24920 1630716..1631066 + 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BCOA_RS24930 1631557..1632384 + 828 WP_014096809.1 RsbT co-antagonist protein RsbRA -
BCOA_RS24935 1632388..1632744 + 357 WP_014096810.1 STAS domain-containing protein -
BCOA_RS24940 1632749..1633150 + 402 WP_014096811.1 anti-sigma regulatory factor -
BCOA_RS24945 1633164..1634174 + 1011 WP_014096812.1 PP2C family protein-serine/threonine phosphatase -
BCOA_RS24950 1634244..1634579 + 336 WP_026104587.1 anti-sigma factor antagonist -
BCOA_RS24955 1634583..1635053 + 471 WP_014096814.1 anti-sigma B factor RsbW -
BCOA_RS24960 1635031..1635810 + 780 WP_014096815.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T25648 WP_013860680.1 NC_016023:1630716-1631066 [Bacillus coagulans 36D1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T25648 NC_016023:1630716-1631066 [Bacillus coagulans 36D1]
TTGATTGTTAAGCGTGGAGACGTGTACTTCGCCGATCTATCTCCGGTGGTCGGCTCCGAGCAGGGAGGCGTACGTCCTGT
CCTCATTTTACAAAACGACATTGGAAACCGTTTTAGCCCCACTGTCATTGTTGCCGCGATAACAGCGCAAATCCAGAAAG
CAAAATTGCCCACGCATGTGGAAATTGATGCAAAGCGTTACGGTTTGGAAAGGGATTCTGTGATATTGCTTGAACAAATA
CGCACAATCGATAAACAACGCTTAACGGATAAGATTACGCATCTTGATGAAGAAGTCATGGAAAAAATTGACGACGCCCT
GCAAATCAGCCTGGGACTGGTCGAATTTTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT25648 WP_014096808.1 NC_016023:1630433-1630711 [Bacillus coagulans 36D1]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT25648 NC_016023:1630433-1630711 [Bacillus coagulans 36D1]
GTGTCTGAACCTATTTCAACAGAAATTTTAGTCCGCTTGCCGCAGCAGCTGTTGACGGAATTGGACGGATTTGCTGAAAC
TGAAAATATGAATCGCAATGAATTAATTTACCGTGCCACAAAAATGTATTTAAAAGAACGGAAAAAACGCCAAATCCGCG
AAACGATGAGGCGTGGTTACATGGAAATGGCAAAAATTAATTTGGCGATTGCCTCCGAAGCGATCCAGGCTGAATACGAA
GCAGAACATACAGTTGAAAGCCTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References