25647

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 890891..891520 Replicon chromosome
Accession NC_016011
Organism Listeria ivanovii subsp. ivanovii PAM 55

Toxin (Protein)


Gene name pemK Uniprot ID A0A097BBV7
Locus tag LIV_RS04240 Protein ID WP_025279828.1
Coordinates 891173..891520 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G2ZDP2
Locus tag LIV_RS04235 Protein ID WP_014092317.1
Coordinates 890891..891169 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIV_RS04215 886229..887701 + 1473 WP_014092313.1 PH domain-containing protein -
LIV_RS04220 887824..889203 + 1380 WP_014092314.1 protoporphyrinogen oxidase -
LIV_RS04225 889205..889561 + 357 WP_014092315.1 holo-ACP synthase -
LIV_RS04230 889579..890685 + 1107 WP_014092316.1 alanine racemase -
LIV_RS04235 890891..891169 + 279 WP_014092317.1 CopG family ribbon-helix-helix protein Antitoxin
LIV_RS04240 891173..891520 + 348 WP_025279828.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LIV_RS04245 891770..892606 + 837 WP_014092318.1 STAS domain-containing protein -
LIV_RS04250 892612..892968 + 357 WP_014092319.1 STAS domain-containing protein -
LIV_RS04255 892971..893381 + 411 WP_014092320.1 anti-sigma regulatory factor -
LIV_RS04260 893398..894402 + 1005 WP_014092321.1 PP2C family protein-serine/threonine phosphatase -
LIV_RS04265 894554..894898 + 345 WP_014092322.1 anti sigma b factor antagonist RsbV -
LIV_RS04270 894882..895355 + 474 WP_014092323.1 anti-sigma B factor RsbW -
LIV_RS04275 895333..896112 + 780 WP_014092324.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12829.90 Da        Isoelectric Point: 6.4735

>T25647 WP_025279828.1 NC_016011:891173-891520 [Listeria ivanovii subsp. ivanovii PAM 55]
MMVKRGDVYYADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAKIQKAKLPTHVEATRKDGFERDSVILLEQIR
TIDKQRLTDKITHLDEELMVKVNQALEVSLGVVEF

Download         Length: 348 bp

>T25647 NC_016011:891173-891520 [Listeria ivanovii subsp. ivanovii PAM 55]
CTGATGGTGAAGCGTGGTGACGTATACTACGCGGACCTTTCCCCCGTGGTCGGAAGCGAGCAAGGGGGAATACGGCCTGT
TCTCATCATTCAAAACGACATTGGTAATAGATTCAGCCCAACTGTGATTGTGGCAGCAATAACCGCAAAGATTCAAAAAG
CAAAGCTGCCAACACATGTGGAAGCTACTCGTAAAGATGGCTTTGAAAGAGATTCTGTCATTCTTTTAGAACAAATTAGA
ACAATTGACAAACAACGCCTGACAGATAAGATTACACATTTGGACGAGGAACTAATGGTCAAGGTAAACCAGGCGCTGGA
AGTCAGTCTAGGAGTAGTAGAATTCTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10621.13 Da        Isoelectric Point: 4.2980

>AT25647 WP_014092317.1 NC_016011:890891-891169 [Listeria ivanovii subsp. ivanovii PAM 55]
VLEKEKRMIISVELTQEMVQELDVVVEKEKMGRSEVIMEATQQFLQEKRARELRDEMERGYAEMATINFAIACECTHVES
EAEDRNISILGG

Download         Length: 279 bp

>AT25647 NC_016011:890891-891169 [Listeria ivanovii subsp. ivanovii PAM 55]
GTGTTAGAGAAAGAAAAGCGGATGATAATATCCGTAGAACTGACACAAGAAATGGTACAGGAACTCGACGTAGTTGTAGA
AAAAGAAAAAATGGGGCGGAGTGAAGTTATAATGGAAGCAACGCAACAATTTTTGCAAGAGAAAAGGGCACGTGAGTTGA
GAGACGAGATGGAGCGCGGCTATGCGGAAATGGCGACAATTAACTTTGCTATCGCATGCGAGTGTACCCATGTCGAATCA
GAAGCAGAAGACAGAAATATTAGTATTTTAGGAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A097BBV7

Antitoxin

Source ID Structure
AlphaFold DB G2ZDP2

References