Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4350198..4350695 Replicon chromosome
Accession NZ_CP103742
Organism Escherichia coli O25b:H4-ST131 strain 2822

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5R22_RS21305 Protein ID WP_001173343.1
Coordinates 4350198..4350371 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag M5R22_RS21310 Protein ID WP_001312331.1
Coordinates 4350399..4350695 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R22_RS21295 (4346868) 4346868..4348325 + 1458 WP_001610404.1 dipeptide/tripeptide permease DtpC -
M5R22_RS21300 (4348562) 4348562..4350079 + 1518 WP_000003806.1 lysine--tRNA ligase -
M5R22_RS21305 (4350198) 4350198..4350371 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5R22_RS21310 (4350399) 4350399..4350695 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5R22_RS21315 (4350923) 4350923..4351195 - 273 WP_000405643.1 GNAT family N-acetyltransferase -
M5R22_RS21320 (4351409) 4351409..4355338 - 3930 WP_001080127.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4350198..4366369 16171


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T256465 WP_001173343.1 NZ_CP103742:c4350371-4350198 [Escherichia coli O25b:H4-ST131]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT256465 WP_001312331.1 NZ_CP103742:c4350695-4350399 [Escherichia coli O25b:H4-ST131]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References