Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4070968..4071544 Replicon chromosome
Accession NZ_CP103742
Organism Escherichia coli O25b:H4-ST131 strain 2822

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5R22_RS19905 Protein ID WP_001297643.1
Coordinates 4071257..4071544 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M5R22_RS19900 Protein ID WP_000063156.1
Coordinates 4070968..4071270 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R22_RS19885 (4067564) 4067564..4069714 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5R22_RS19890 (4069764) 4069764..4069967 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5R22_RS19895 (4069978) 4069978..4070934 + 957 WP_001297640.1 GTPase -
M5R22_RS19900 (4070968) 4070968..4071270 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M5R22_RS19905 (4071257) 4071257..4071544 - 288 WP_001297643.1 BrnT family toxin Toxin
M5R22_RS19910 (4071743) 4071743..4072440 - 698 Protein_3899 IS1 family transposase -
M5R22_RS19915 (4072497) 4072497..4072592 + 96 Protein_3900 dienelactone hydrolase family protein -
M5R22_RS19920 (4072738) 4072738..4073970 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
M5R22_RS19925 (4074011) 4074011..4075291 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4071743..4071952 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T256463 WP_001297643.1 NZ_CP103742:c4071544-4071257 [Escherichia coli O25b:H4-ST131]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT256463 WP_000063156.1 NZ_CP103742:c4071270-4070968 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References