Detailed information of TA system TA256461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3731722..3733411
• Replicon: chromosome
• Accession: NZ_CP103742
• Organism: Escherichia coli O25b:H4-ST131 strain 2822

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: M5R22_RS18285
• Protein ID: WP_000365807.1
• Coordinates: 3731722..3732642 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: M5R22_RS18290
• Protein ID: WP_001295795.1
• Coordinates: 3732629..3733411 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5R22_RS18245 (3727195)	3727195..3727905	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
M5R22_RS18250 (3728212)	3728212..3728541	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
M5R22_RS18255 (3728553)	3728553..3728719	-	167	Protein_3579	tail fiber assembly protein	-
M5R22_RS18260 (3728790)	3728790..3729404	-	615	WP_001019923.1	YagU family protein	-
M5R22_RS18265 (3729619)	3729619..3729804	-	186	WP_000146240.1	hypothetical protein	-
M5R22_RS18270 (3729853)	3729853..3730011	+	159	Protein_3582	integrase	-
M5R22_RS18275 (3730238)	3730238..3730573	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
M5R22_RS18280 (3730781)	3730781..3731557	+	777	WP_000171155.1	site-specific integrase	-
M5R22_RS18285 (3731722)	3731722..3732642	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M5R22_RS18290 (3732629)	3732629..3733411	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
M5R22_RS18295 (3733695)	3733695..3736793	+	3099	WP_000673070.1	type I restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3691509..3740474	48965
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3692549..3740474	47925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T256461 WP_000365807.1 NZ_CP103742:c3732642-3731722 [Escherichia coli O25b:H4-ST131]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT256461 WP_001295795.1 NZ_CP103742:c3733411-3732629 [Escherichia coli O25b:H4-ST131]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0