Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3547656..3548274 Replicon chromosome
Accession NZ_CP103742
Organism Escherichia coli O25b:H4-ST131 strain 2822

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag M5R22_RS17415 Protein ID WP_001291435.1
Coordinates 3548056..3548274 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag M5R22_RS17410 Protein ID WP_000344800.1
Coordinates 3547656..3548030 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R22_RS17400 (3542745) 3542745..3543938 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
M5R22_RS17405 (3543961) 3543961..3547110 + 3150 WP_001132478.1 efflux RND transporter permease AcrB -
M5R22_RS17410 (3547656) 3547656..3548030 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
M5R22_RS17415 (3548056) 3548056..3548274 + 219 WP_001291435.1 HHA domain-containing protein Toxin
M5R22_RS17420 (3548447) 3548447..3548998 + 552 WP_000102539.1 maltose O-acetyltransferase -
M5R22_RS17425 (3549114) 3549114..3549584 + 471 WP_000136192.1 YlaC family protein -
M5R22_RS17430 (3549748) 3549748..3551298 + 1551 WP_001545832.1 cyclic-guanylate-specific phosphodiesterase PdeB -
M5R22_RS17435 (3551340) 3551340..3551693 - 354 WP_000878147.1 DUF1428 family protein -
M5R22_RS17445 (3552072) 3552072..3552383 + 312 WP_000409908.1 MGMT family protein -
M5R22_RS17450 (3552414) 3552414..3552986 - 573 WP_000779841.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T256460 WP_001291435.1 NZ_CP103742:3548056-3548274 [Escherichia coli O25b:H4-ST131]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT256460 WP_000344800.1 NZ_CP103742:3547656-3548030 [Escherichia coli O25b:H4-ST131]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References