Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2843413..2843884 Replicon chromosome
Accession NZ_CP103742
Organism Escherichia coli O25b:H4-ST131 strain 2822

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag M5R22_RS13925 Protein ID WP_001303511.1
Coordinates 2843606..2843884 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag M5R22_RS13920 Protein ID WP_001302048.1
Coordinates 2843413..2843604 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R22_RS13875 (2838662) 2838662..2838880 - 219 WP_001610644.1 DUF4014 family protein -
M5R22_RS13880 (2838913) 2838913..2839125 - 213 WP_000072553.1 hypothetical protein -
M5R22_RS13885 (2839231) 2839231..2839653 - 423 Protein_2723 DUF977 family protein -
M5R22_RS13890 (2839669) 2839669..2840439 - 771 WP_000450998.1 DUF1627 domain-containing protein -
M5R22_RS13895 (2840461) 2840461..2841207 - 747 WP_000788950.1 ATP-binding protein -
M5R22_RS13900 (2841214) 2841214..2842176 - 963 WP_000095673.1 helix-turn-helix domain-containing protein -
M5R22_RS13905 (2842199) 2842199..2842624 - 426 WP_000693943.1 toxin YdaT family protein -
M5R22_RS13910 (2842621) 2842621..2842923 - 303 WP_001556930.1 transcriptional regulator -
M5R22_RS13915 (2843021) 2843021..2843392 + 372 WP_001169687.1 hypothetical protein -
M5R22_RS13920 (2843413) 2843413..2843604 + 192 WP_001302048.1 hypothetical protein Antitoxin
M5R22_RS13925 (2843606) 2843606..2843884 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5R22_RS13930 (2844175) 2844175..2844327 + 153 WP_000380313.1 DUF1391 family protein -
M5R22_RS13935 (2844339) 2844339..2844677 + 339 WP_000394541.1 hypothetical protein -
M5R22_RS13940 (2844666) 2844666..2844860 - 195 WP_001295058.1 hypothetical protein -
M5R22_RS13945 (2845436) 2845436..2845624 + 189 WP_000449175.1 cell division inhibition protein DicB -
M5R22_RS13950 (2845621) 2845621..2845809 + 189 WP_000199475.1 DUF1482 family protein -
M5R22_RS13955 (2845902) 2845902..2848340 + 2439 WP_000102132.1 exonuclease -
M5R22_RS13960 (2848402) 2848402..2848671 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2760263..2856926 96663


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T256458 WP_001303511.1 NZ_CP103742:2843606-2843884 [Escherichia coli O25b:H4-ST131]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT256458 WP_001302048.1 NZ_CP103742:2843413-2843604 [Escherichia coli O25b:H4-ST131]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References