Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4001227..4001881 Replicon chromosome
Accession NZ_CP103739
Organism Escherichia coli strain 3359

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag M5T33_RS19690 Protein ID WP_000244781.1
Coordinates 4001227..4001634 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag M5T33_RS19695 Protein ID WP_000354046.1
Coordinates 4001615..4001881 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T33_RS19670 (3997184) 3997184..3998917 - 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -
M5T33_RS19675 (3998923) 3998923..3999633 - 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
M5T33_RS19680 (3999658) 3999658..4000554 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
M5T33_RS19685 (4000666) 4000666..4001187 + 522 WP_001055874.1 flavodoxin FldB -
M5T33_RS19690 (4001227) 4001227..4001634 - 408 WP_000244781.1 protein YgfX Toxin
M5T33_RS19695 (4001615) 4001615..4001881 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
M5T33_RS19700 (4002124) 4002124..4003104 + 981 WP_000886088.1 tRNA-modifying protein YgfZ -
M5T33_RS19705 (4003181) 4003181..4003840 - 660 WP_000250274.1 hemolysin III family protein -
M5T33_RS19710 (4004004) 4004004..4004315 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
M5T33_RS19715 (4004360) 4004360..4005793 + 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T256437 WP_000244781.1 NZ_CP103739:c4001634-4001227 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT256437 WP_000354046.1 NZ_CP103739:c4001881-4001615 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References