Detailed information of TA system TA025643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 3318120..3318685
• Replicon: chromosome
• Accession: NC_015977
• Organism: Roseburia hominis A2-183

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G2T4T1
• Locus tag: RHOM_RS15080
• Protein ID: WP_014081142.1
• Coordinates: 3318395..3318685 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G2T4T0
• Locus tag: RHOM_RS15075
• Protein ID: WP_014081141.1
• Coordinates: 3318120..3318398 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RHOM_RS15035	3313673..3313882	-	210	WP_015514784.1	helix-turn-helix transcriptional regulator	-
RHOM_RS15040	3314019..3314177	-	159	WP_014081135.1	membrane protein	-
RHOM_RS15045	3314285..3315097	-	813	WP_080633471.1	TraM recognition domain-containing protein	-
RHOM_RS15050	3315206..3315571	-	366	WP_014081136.1	hypothetical protein	-
RHOM_RS15055	3315649..3315843	-	195	WP_014081137.1	transposon-encoded TnpW family protein	-
RHOM_RS15060	3315887..3316750	-	864	WP_014081138.1	ATP-binding protein	-
RHOM_RS15065	3316747..3317481	-	735	WP_014081139.1	phage replisome organizer N-terminal domain-containing protein	-
RHOM_RS15070	3317595..3317993	-	399	WP_014081140.1	cysteine-rich VLP domain-containing protein	-
RHOM_RS15075	3318120..3318398	+	279	WP_014081141.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
RHOM_RS15080	3318395..3318685	+	291	WP_014081142.1	type II toxin-antitoxin system YafQ family toxin	Toxin
RHOM_RS15085	3318763..3320208	-	1446	WP_014081143.1	MobA/MobL family protein	-
RHOM_RS15090	3320423..3320713	-	291	WP_014081144.1	DUF3847 domain-containing protein	-
RHOM_RS15095	3320742..3321203	-	462	WP_009296689.1	sigma-70 family RNA polymerase sigma factor	-
RHOM_RS15100	3321214..3321666	-	453	WP_014081145.1	hypothetical protein	-
RHOM_RS15105	3321770..3322183	-	414	WP_014081146.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3322559..3323749	1190

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11153.97 Da
• Isoelectric Point: 9.4307

>T25643 WP_014081142.1 NC_015977:3318395-3318685 [Roseburia hominis A2-183]
MRKTKYTVKYTTAFKKDYKRAIKRGLKIELLEQVVALLSMGEPLPDKNRDHDLSGDWAGHRECHILPDWLLVYRIEEDVL
VLTLARTGTHSDLFGK

Nucleotide

• Length: 291 bp

>T25643 NC_015977:3318395-3318685 [Roseburia hominis A2-183]
ATGAGGAAAACAAAGTACACCGTCAAGTACACGACCGCTTTCAAAAAGGACTACAAGCGGGCAATCAAGCGCGGCCTGAA
AATCGAATTGCTGGAACAGGTCGTGGCGCTGCTGTCGATGGGCGAACCGCTGCCGGATAAAAACCGCGATCACGATTTGT
CCGGCGATTGGGCAGGCCATCGAGAATGTCATATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGAGGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10233.68 Da
• Isoelectric Point: 4.4692

>AT25643 WP_014081141.1 NC_015977:3318120-3318398 [Roseburia hominis A2-183]
MAGNTTNISIRMDADLKAQADALFTELGMNLTTAFNIFVRQSLREGGIPFEVRLEQPNKETIAAMLEAERIAKDPSVKGY
NDLDELFADLKK

Nucleotide

• Length: 279 bp

>AT25643 NC_015977:3318120-3318398 [Roseburia hominis A2-183]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAGCT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAGGAAACGATTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGATAC
AATGACCTTGACGAGTTGTTTGCCGACCTGAAAAAATGA

Structures

Toxin

• AlphaFold DB: G2T4T1

Antitoxin

• AlphaFold DB: A0A6L8TBH6