Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 821068..821644 Replicon chromosome
Accession NZ_CP103739
Organism Escherichia coli strain 3359

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5T33_RS04030 Protein ID WP_001297643.1
Coordinates 821068..821355 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M5T33_RS04035 Protein ID WP_000063156.1
Coordinates 821342..821644 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T33_RS04010 (817321) 817321..818601 - 1281 WP_001037380.1 DUF445 domain-containing protein -
M5T33_RS04015 (818642) 818642..819874 - 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
M5T33_RS04020 (820020) 820020..820115 - 96 Protein_774 dienelactone hydrolase family protein -
M5T33_RS04025 (820172) 820172..820869 + 698 Protein_775 IS1 family transposase -
M5T33_RS04030 (821068) 821068..821355 + 288 WP_001297643.1 BrnT family toxin Toxin
M5T33_RS04035 (821342) 821342..821644 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M5T33_RS04040 (821678) 821678..822634 - 957 WP_001297640.1 GTPase -
M5T33_RS04045 (822645) 822645..822848 - 204 WP_000467859.1 YbdD/YjiX family protein -
M5T33_RS04050 (822898) 822898..825048 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T256423 WP_001297643.1 NZ_CP103739:821068-821355 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT256423 WP_000063156.1 NZ_CP103739:821342-821644 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References