Detailed information of TA system TA025642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 2265366..2265931
• Replicon: chromosome
• Accession: NC_015977
• Organism: Roseburia hominis A2-183

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C7H6P5
• Locus tag: RHOM_RS10190
• Protein ID: WP_002596328.1
• Coordinates: 2265641..2265931 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C7H6P4
• Locus tag: RHOM_RS10185
• Protein ID: WP_005924829.1
• Coordinates: 2265366..2265644 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RHOM_RS10145	2260507..2260995	-	489	WP_002569239.1	hypothetical protein	-
RHOM_RS17005	2261220..2261360	-	141	WP_014080223.1	membrane protein	-
RHOM_RS10155	2261456..2262343	-	888	Protein_1995	TraM recognition domain-containing protein	-
RHOM_RS10160	2262437..2262802	-	366	WP_014080225.1	hypothetical protein	-
RHOM_RS10165	2262880..2263074	-	195	WP_005933543.1	transposon-encoded TnpW family protein	-
RHOM_RS10170	2263118..2263981	-	864	WP_005933545.1	ATP-binding protein	-
RHOM_RS10175	2263978..2264727	-	750	WP_005933547.1	phage replisome organizer N-terminal domain-containing protein	-
RHOM_RS10180	2264841..2265239	-	399	WP_005933550.1	cysteine-rich VLP domain-containing protein	-
RHOM_RS10185	2265366..2265644	+	279	WP_005924829.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
RHOM_RS10190	2265641..2265931	+	291	WP_002596328.1	type II toxin-antitoxin system YafQ family toxin	Toxin
RHOM_RS10195	2266039..2267484	-	1446	WP_014080226.1	MobA/MobL family protein	-
RHOM_RS10200	2267670..2267960	-	291	WP_014080227.1	DUF3847 domain-containing protein	-
RHOM_RS10205	2267989..2268450	-	462	WP_014080228.1	sigma-70 family RNA polymerase sigma factor	-
RHOM_RS10210	2268461..2268913	-	453	WP_014080229.1	sigma-24 (feci)	-
RHOM_RS10215	2269017..2269430	-	414	WP_014080230.1	hypothetical protein	-
RHOM_RS10220	2269885..2270346	-	462	WP_044024973.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2232358..2279371	47013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-94)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-90)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11112.87 Da
• Isoelectric Point: 8.0630

>T25642 WP_002596328.1 NC_015977:2265641-2265931 [Roseburia hominis A2-183]
MKETKYTVKYTTSFKKDYKRAIKRGLKIELLEQVVALLAMGEPLPDKNRDHDLSGDWAGHRECHILPDWLLVYRIEDDVL
VLTLARTGTHSDLFGK

Nucleotide

• Length: 291 bp

>T25642 NC_015977:2265641-2265931 [Roseburia hominis A2-183]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAGGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATTGAGCTGCTGGAGCAGGTCGTAGCATTGCTGGCAATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGTCATCGGGAATGTCATATTCTCCCGGACTGGCTGCTTGTCTATCGCATAGAGGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10247.66 Da
• Isoelectric Point: 4.4692

>AT25642 WP_005924829.1 NC_015977:2265366-2265644 [Roseburia hominis A2-183]
MAGNTTNISIRMDADLKAQADALFTELGMNLTTAFNIFVRQSLREGGIPFEVRLEQPNKETVAAMLEAERIAKDPSVKGY
NDLDELFADLKR

Nucleotide

• Length: 279 bp

>AT25642 NC_015977:2265366-2265644 [Roseburia hominis A2-183]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACGGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA

Structures

Toxin

• AlphaFold DB: A0A1M4XBM1

Antitoxin

• AlphaFold DB: A0A1M4XB19