Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5231867..5232492 Replicon chromosome
Accession NZ_CP103729
Organism Klebsiella pneumoniae strain 197

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M5R33_RS25750 Protein ID WP_002882817.1
Coordinates 5231867..5232250 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M5R33_RS25755 Protein ID WP_004150355.1
Coordinates 5232250..5232492 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R33_RS25735 (5229233) 5229233..5230135 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M5R33_RS25740 (5230132) 5230132..5230767 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M5R33_RS25745 (5230764) 5230764..5231693 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M5R33_RS25750 (5231867) 5231867..5232250 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M5R33_RS25755 (5232250) 5232250..5232492 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M5R33_RS25760 (5232697) 5232697..5233614 + 918 WP_064150666.1 alpha/beta hydrolase -
M5R33_RS25765 (5233629) 5233629..5234570 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M5R33_RS25770 (5234615) 5234615..5235052 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M5R33_RS25775 (5235049) 5235049..5235909 - 861 WP_004146232.1 virulence factor BrkB family protein -
M5R33_RS25780 (5235903) 5235903..5236502 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T256400 WP_002882817.1 NZ_CP103729:c5232250-5231867 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT256400 WP_004150355.1 NZ_CP103729:c5232492-5232250 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References