Detailed information of TA system TA025640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2601249..2601823
• Replicon: chromosome
• Accession: NC_015976
• Organism: Sphingobium sp. SYK-6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G2IIH0
• Locus tag: SLG_RS11990
• Protein ID: WP_014076738.1
• Coordinates: 2601249..2601581 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: SLG_RS11995
• Protein ID: WP_041391978.1
• Coordinates: 2601581..2601823 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SLG_RS11975	2596570..2598606	+	2037	WP_014076735.1	RNA polymerase sigma factor RpoD	-
SLG_RS11980	2598699..2599991	-	1293	WP_014076736.1	glycosyltransferase family 4 protein	-
SLG_RS11985	2600344..2601024	+	681	WP_041391976.1	hypothetical protein	-
SLG_RS11990	2601249..2601581	-	333	WP_014076738.1	endoribonuclease MazF	Toxin
SLG_RS11995	2601581..2601823	-	243	WP_041391978.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SLG_RS12000	2601992..2603197	-	1206	WP_014076740.1	DUF1343 domain-containing protein	-
SLG_RS12005	2603194..2603748	-	555	WP_014076741.1	DOMON-like domain-containing protein	-
SLG_RS12010	2603810..2605042	+	1233	WP_014076742.1	tyrosine--tRNA ligase	-
SLG_RS12015	2605150..2605470	+	321	WP_014076743.1	PilZ domain-containing protein	-
SLG_RS12020	2605558..2605923	+	366	WP_014076744.1	PilZ domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11974.95 Da
• Isoelectric Point: 9.1504

>T25640 WP_014076738.1 NC_015976:c2601581-2601249 [Sphingobium sp. SYK-6]
VGAYVPELGDIVWLHFDPQAGHEQAGHRPAVVLSPASYNRARGMMICCPMTSRIKGYPFEVVVSETPPSAVLADQIKSLD
WRARLASRKGAVPQGVLVEVQAKIRALLNL

Nucleotide

• Length: 333 bp

>T25640 NC_015976:c2601581-2601249 [Sphingobium sp. SYK-6]
GTGGGGGCTTATGTTCCGGAGCTCGGCGATATTGTCTGGCTTCATTTCGACCCTCAGGCGGGCCATGAGCAAGCGGGCCA
TCGCCCGGCCGTTGTGCTGTCGCCGGCTTCGTACAATCGTGCGCGCGGGATGATGATCTGCTGTCCGATGACGTCGAGGA
TAAAAGGATATCCGTTCGAAGTCGTCGTGAGCGAGACTCCGCCAAGCGCGGTGCTGGCCGATCAGATCAAGAGTCTCGAC
TGGCGGGCTCGTCTCGCTTCCAGGAAAGGCGCGGTGCCACAGGGTGTGCTGGTTGAGGTTCAGGCGAAAATTCGTGCTTT
GCTGAACCTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8841.98 Da
• Isoelectric Point: 4.2950

>AT25640 WP_041391978.1 NC_015976:c2601823-2601581 [Sphingobium sp. SYK-6]
MRVHVKKWGNSASVRIPASIMASASLRIDQEVDVREEEGRIIIEPVSTPSYDLDSLLSGMTPDTFPEEVDFGPPAGKEVW

Nucleotide

• Length: 243 bp

>AT25640 NC_015976:c2601823-2601581 [Sphingobium sp. SYK-6]
ATGCGTGTTCATGTCAAGAAATGGGGCAATAGCGCCTCGGTGAGAATCCCGGCCTCGATCATGGCATCGGCATCTCTGCG
CATCGATCAGGAGGTGGATGTCCGCGAGGAAGAGGGACGCATCATCATCGAGCCGGTTTCCACGCCTTCCTATGACCTGG
ACTCGCTGCTCAGTGGCATGACGCCTGACACCTTCCCGGAAGAAGTCGACTTTGGTCCGCCGGCAGGCAAGGAAGTCTGG
TAG

Structures

Toxin

• AlphaFold DB: G2IIH0