Detailed information of TA system TA256399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4877400..4879081
• Replicon: chromosome
• Accession: NZ_CP103729
• Organism: Klebsiella pneumoniae strain 197

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: M5R33_RS24120
• Protein ID: WP_004146663.1
• Coordinates: 4877400..4878317 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: M5R33_RS24125
• Protein ID: WP_004177809.1
• Coordinates: 4878323..4879081 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5R33_RS24100 (4872706)	4872706..4874226	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
M5R33_RS24105 (4874223)	4874223..4875221	+	999	WP_002885172.1	ribose ABC transporter permease	-
M5R33_RS24110 (4875252)	4875252..4876193	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
M5R33_RS24115 (4876268)	4876268..4877275	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
M5R33_RS24120 (4877400)	4877400..4878317	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M5R33_RS24125 (4878323)	4878323..4879081	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
M5R33_RS24130 (4879167)	4879167..4879895	-	729	WP_114265984.1	response regulator transcription factor	-
M5R33_RS24135 (4880168)	4880168..4882315	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
M5R33_RS24140 (4882545)	4882545..4883231	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T256399 WP_004146663.1 NZ_CP103729:c4878317-4877400 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT256399 WP_004177809.1 NZ_CP103729:c4879081-4878323 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05