Detailed information of TA system TA025639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 96223..96766
• Replicon: chromosome
• Accession: NC_015975
• Organism: Lactobacillus ruminis ATCC 27782

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G2SPY9
• Locus tag: LRC_RS00430
• Protein ID: WP_014072699.1
• Coordinates: 96479..96766 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G2SPY8
• Locus tag: LRC_RS00425
• Protein ID: WP_003697732.1
• Coordinates: 96223..96495 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRC_RS00410	91509..92270	+	762	WP_041819731.1	DeoR/GlpR family DNA-binding transcription regulator	-
LRC_RS00415	92267..93181	+	915	WP_014072697.1	1-phosphofructokinase	-
LRC_RS00420	93196..95157	+	1962	WP_014072698.1	fructose-specific PTS transporter subunit EIIC	-
LRC_RS11455	95978..96124	-	147	WP_167317005.1	hypothetical protein	-
LRC_RS00425	96223..96495	+	273	WP_003697732.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LRC_RS00430	96479..96766	+	288	WP_014072699.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LRC_RS00435	97221..97430	+	210	WP_041819734.1	hypothetical protein	-
LRC_RS00440	97458..98081	-	624	WP_014072700.1	RloB family protein	-
LRC_RS00445	98082..99341	-	1260	WP_014072701.1	ATP-binding protein	-
LRC_RS00450	99509..100408	-	900	WP_014072702.1	hypothetical protein	-
LRC_RS00455	100797..101585	+	789	WP_014072703.1	chain-length determining protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (7-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-88)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11276.06 Da
• Isoelectric Point: 9.4201

>T25639 WP_014072699.1 NC_015975:96479-96766 [Lactobacillus ruminis ATCC 27782]
MRLKNKYDIQFTGQFKRDFKLAKKQHRNLDRLFKVIDVLAAGGTLDEKYRDHALTGNYKGTRECHVEPDCLLIYEIRQDV
LVLFLYRLGTHSELF

Nucleotide

• Length: 288 bp

>T25639 NC_015975:96479-96766 [Lactobacillus ruminis ATCC 27782]
ATGCGCTTGAAAAATAAGTACGATATTCAGTTCACCGGTCAGTTTAAGAGGGATTTCAAGCTTGCGAAAAAGCAGCATAG
GAATCTTGATAGATTGTTTAAAGTGATTGATGTTTTAGCGGCTGGCGGCACATTGGACGAAAAGTACAGAGATCATGCTC
TTACGGGTAATTACAAGGGCACACGCGAATGTCACGTTGAACCGGATTGCCTGCTCATCTATGAAATTCGCCAAGACGTG
CTGGTCCTGTTTCTTTACAGGCTAGGCACACATTCTGAATTGTTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10182.57 Da
• Isoelectric Point: 4.8896

>AT25639 WP_003697732.1 NC_015975:96223-96495 [Lactobacillus ruminis ATCC 27782]
MSTTNLNIRTDKDVKEKAEKIFNELGLNMTSAVNIFLRTAIRERGIPFELKLDVPNETTIAAIEEGRKMASDPSSPRYSN
IDELRDALEK

Nucleotide

• Length: 273 bp

>AT25639 NC_015975:96223-96495 [Lactobacillus ruminis ATCC 27782]
ATGTCAACAACGAATTTAAACATCAGAACAGATAAGGATGTCAAAGAGAAAGCAGAGAAAATTTTCAATGAACTTGGATT
AAACATGACCAGTGCCGTAAATATTTTTCTGAGAACTGCTATTCGTGAACGCGGCATTCCGTTTGAATTGAAATTGGACG
TGCCGAACGAAACGACGATTGCAGCGATTGAAGAGGGGAGGAAAATGGCTTCCGATCCATCTTCACCAAGATATTCCAAC
ATTGATGAATTGAGGGATGCGCTTGAAAAATAA

Structures

Toxin

• AlphaFold DB: G2SPY9

Antitoxin

• AlphaFold DB: G2SPY8