Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 11120..11763 Replicon plasmid pMB3028_1
Accession NZ_CP103728
Organism Klebsiella pneumoniae strain 2289

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag M5T50_RS25335 Protein ID WP_016236302.1
Coordinates 11347..11763 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag M5T50_RS25330 Protein ID WP_001261282.1
Coordinates 11120..11350 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T50_RS25305 (M5T50_25305) 6835..7092 - 258 WP_023292103.1 hypothetical protein -
M5T50_RS25310 (M5T50_25310) 7571..8299 + 729 Protein_6 CSS-motif domain-containing protein -
M5T50_RS25315 (M5T50_25315) 8315..9151 + 837 WP_032439674.1 EAL domain-containing protein -
M5T50_RS25320 (M5T50_25320) 9769..10200 - 432 WP_174805835.1 hypothetical protein -
M5T50_RS25325 (M5T50_25325) 10741..11163 - 423 WP_046624301.1 hypothetical protein -
M5T50_RS25330 (M5T50_25330) 11120..11350 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M5T50_RS25335 (M5T50_25335) 11347..11763 + 417 WP_016236302.1 type II toxin-antitoxin system VapC family toxin Toxin
M5T50_RS25340 (M5T50_25340) 11837..13399 + 1563 WP_004206609.1 AAA family ATPase -
M5T50_RS25345 (M5T50_25345) 13384..14406 + 1023 WP_032439672.1 helicase UvrD -
M5T50_RS25350 (M5T50_25350) 14951..15859 + 909 WP_032439686.1 HNH endonuclease -
M5T50_RS25355 (M5T50_25355) 16045..16395 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / dfrA14 / aac(3)-IIa / qnrB1 / tet(A) - 1..152422 152422


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15091.55 Da        Isoelectric Point: 7.1084

>T256386 WP_016236302.1 NZ_CP103728:11347-11763 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT256386 WP_001261282.1 NZ_CP103728:11120-11350 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References