Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5084512..5085137 Replicon chromosome
Accession NZ_CP103727
Organism Klebsiella pneumoniae strain 2289

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M5T50_RS24915 Protein ID WP_002882817.1
Coordinates 5084512..5084895 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M5T50_RS24920 Protein ID WP_004150355.1
Coordinates 5084895..5085137 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T50_RS24900 (5081878) 5081878..5082780 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M5T50_RS24905 (5082777) 5082777..5083412 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M5T50_RS24910 (5083409) 5083409..5084338 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M5T50_RS24915 (5084512) 5084512..5084895 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M5T50_RS24920 (5084895) 5084895..5085137 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M5T50_RS24925 (5085342) 5085342..5086259 + 918 WP_023302328.1 alpha/beta hydrolase -
M5T50_RS24930 (5086273) 5086273..5087214 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M5T50_RS24935 (5087259) 5087259..5087696 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M5T50_RS24940 (5087693) 5087693..5088553 - 861 WP_004146232.1 virulence factor BrkB family protein -
M5T50_RS24945 (5088547) 5088547..5089146 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T256385 WP_002882817.1 NZ_CP103727:c5084895-5084512 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT256385 WP_004150355.1 NZ_CP103727:c5085137-5084895 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References