Detailed information of TA system TA256384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4766137..4767818
• Replicon: chromosome
• Accession: NZ_CP103727
• Organism: Klebsiella pneumoniae strain 2289

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: M5T50_RS23460
• Protein ID: WP_004146663.1
• Coordinates: 4766137..4767054 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: M5T50_RS23465
• Protein ID: WP_004177809.1
• Coordinates: 4767060..4767818 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5T50_RS23440 (4761443)	4761443..4762963	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
M5T50_RS23445 (4762960)	4762960..4763958	+	999	WP_012737175.1	ribose ABC transporter permease	-
M5T50_RS23450 (4763989)	4763989..4764930	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
M5T50_RS23455 (4765005)	4765005..4766012	+	1008	WP_023302355.1	carbohydrate kinase family protein	-
M5T50_RS23460 (4766137)	4766137..4767054	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M5T50_RS23465 (4767060)	4767060..4767818	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
M5T50_RS23470 (4767904)	4767904..4768632	-	729	WP_002885152.1	response regulator transcription factor	-
M5T50_RS23475 (4768905)	4768905..4771052	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
M5T50_RS23480 (4771282)	4771282..4771968	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T256384 WP_004146663.1 NZ_CP103727:c4767054-4766137 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT256384 WP_004177809.1 NZ_CP103727:c4767818-4767060 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05