Detailed information of TA system TA025638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 71961..72492
• Replicon: plasmid pSLPG
• Accession: NC_015974
• Organism: Sphingobium sp. SYK-6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T0GNF5
• Locus tag: SLG_RS19765
• Protein ID: WP_004212690.1
• Coordinates: 72211..72492 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: T0G968
• Locus tag: SLG_RS19760
• Protein ID: WP_004212689.1
• Coordinates: 71961..72224 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SLG_RS19740	67135..68841	+	1707	WP_007683343.1	copper resistance system multicopper oxidase	-
SLG_RS19745	68838..69824	+	987	WP_009823975.1	copper resistance protein B	-
SLG_RS19750	69818..71059	+	1242	WP_007683345.1	MFS transporter	-
SLG_RS19755	71139..71576	+	438	WP_007683347.1	metal-binding protein	-
SLG_RS19760	71961..72224	+	264	WP_004212689.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SLG_RS19765	72211..72492	+	282	WP_004212690.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SLG_RS19770	72858..73208	+	351	WP_007683352.1	magnesium transporter	-
SLG_RS19775	73271..73678	-	408	WP_007683354.1	response regulator	-
SLG_RS19780	73760..74383	-	624	WP_007683357.1	response regulator transcription factor	-
SLG_RS19785	74373..75908	-	1536	WP_007683359.1	PAS domain S-box protein	-
SLG_RS19790	75967..76440	-	474	WP_007683361.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..148801	148801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10993.49 Da
• Isoelectric Point: 7.4752

>T25638 WP_004212690.1 NC_015974:72211-72492 [Sphingobium sp. SYK-6]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Nucleotide

• Length: 282 bp

>T25638 NC_015974:72211-72492 [Sphingobium sp. SYK-6]
ATGCGGACGATTGAACGCTTCGGGCGGTTCAAGCGCGACTACAAGCGGGAGAAGAAGGGGCAGCACGCCAAGACGCTGGA
CGCTGACCTGATCCCGATCATCGAGGCGCTGGCATCCGACGAGCCGCTTGAACCGCGCCACCGCGACCATGCGCTGACCG
GCGACTGGCGCGACCATCGCGATTGCCACATAAAGCCCGACCTCGTGCTGATTTACCGCAAGCCCGATGACGACACCTTG
CAGCTTGTGCGCTTGGGATCGCACGCGGAATTGGGTTGGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9284.75 Da
• Isoelectric Point: 4.5881

>AT25638 WP_004212689.1 NC_015974:71961-72224 [Sphingobium sp. SYK-6]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT25638 NC_015974:71961-72224 [Sphingobium sp. SYK-6]
ATGGCCGCAAACGCTCTTGTGCAGACCCGGATCGACGGAGCGGTGAAGGAGGAAGCCGCGACCGTGCTGGCTGCTATGGG
GCTGACCGTCTCCGATGCCGTGCGCCTGATGCTGACCCGCGTTGCCCGCGACAAGGCCTTGCCGTTTGAACCGCTGGTGC
CCAATGCCGAGACGATCGAGGCCATGAAGGAAGCGCGCGAGGGAAAGGGCAAGCGGTTCGCCACGGTGGTCGACCTGATG
GCCGACCTCAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: T0GNF5

Antitoxin

• AlphaFold DB: T0G968