Detailed information of TA system TA025637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4712602..4713218
• Replicon: chromosome
• Accession: NC_015968
• Organism: Enterobacter asburiae LF7a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ENTAS_RS22270
• Protein ID: WP_014072389.1
• Coordinates: 4712602..4712973 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A198H0E5
• Locus tag: ENTAS_RS22275
• Protein ID: WP_014072390.1
• Coordinates: 4712976..4713218 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENTAS_RS22255	4710102..4711004	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
ENTAS_RS22260	4711001..4711636	+	636	WP_014072387.1	formate dehydrogenase cytochrome b556 subunit	-
ENTAS_RS22265	4711633..4712562	+	930	WP_014072388.1	formate dehydrogenase accessory protein FdhE	-
ENTAS_RS22270	4712602..4712973	-	372	WP_014072389.1	type II toxin-antitoxin system VapC family toxin	Toxin
ENTAS_RS22275	4712976..4713218	-	243	WP_014072390.1	ribbon-helix-helix protein, CopG family	Antitoxin
ENTAS_RS22280	4713418..4714335	+	918	WP_014072391.1	alpha/beta hydrolase	-
ENTAS_RS22285	4714352..4715293	-	942	WP_041162159.1	fatty acid biosynthesis protein FabY	-
ENTAS_RS22290	4715338..4715775	-	438	WP_014072393.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ENTAS_RS22295	4715772..4716653	-	882	WP_014072394.1	virulence factor BrkB family protein	-
ENTAS_RS22300	4716647..4717246	-	600	WP_014072395.1	glucose-1-phosphatase	-
ENTAS_RS22305	4717377..4718165	-	789	WP_014072396.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13695.79 Da
• Isoelectric Point: 6.9794

>T25637 WP_014072389.1 NC_015968:c4712973-4712602 [Enterobacter soli]
VEHMAVFDTNILIDLFNNRTEAANAIENTASRRAISLITWMEVMVGARRHGHEAKTAAVMRAFEIIDVSRDIAERSVLLR
EEHGMKLPDAIILATAQSRNCPLISRNTKDFAGIVGVISPYHL

Nucleotide

• Length: 372 bp

>T25637 NC_015968:c4712973-4712602 [Enterobacter soli]
GTGGAACACATGGCCGTGTTTGATACCAACATTCTTATCGATCTCTTTAATAATCGCACTGAGGCAGCGAATGCGATTGA
AAACACAGCATCGCGCCGGGCGATCAGCCTGATTACCTGGATGGAAGTGATGGTCGGTGCGCGCAGGCACGGTCATGAAG
CAAAAACGGCGGCTGTGATGAGGGCTTTTGAGATTATCGATGTGTCGCGTGATATCGCGGAAAGAAGCGTTTTACTCCGT
GAGGAACACGGGATGAAACTGCCTGATGCCATCATTCTGGCCACAGCCCAGAGTAGAAATTGCCCATTGATTTCACGTAA
TACGAAAGATTTTGCGGGAATTGTTGGGGTTATCTCACCCTATCATCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9468.69 Da
• Isoelectric Point: 4.2463

>AT25637 WP_014072390.1 NC_015968:c4713218-4712976 [Enterobacter soli]
MSLLVNLDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIEQYLDQQSTSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT25637 NC_015968:c4713218-4712976 [Enterobacter soli]
ATGAGTCTTTTGGTGAACCTTGATATGGGCAGAATACTGATCGATTTGTCGGATGATGTCATTCAACGGCTGGATAACCT
CAAACAGCTGCGGAATCAACCTCGTGCCGAATTGCTGCGCGAAGCGATAGAACAGTATCTCGACCAGCAAAGCACTTCGG
TTATTCGTGATGCGCTGGGTCTGTGGGGGAACCAGCAAGAAGATGGGCTTGAGTACGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A198H0E5