Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2946214..2946804 Replicon chromosome
Accession NC_015968
Organism Enterobacter asburiae LF7a

Toxin (Protein)


Gene name pemK Uniprot ID A0A198GGF1
Locus tag ENTAS_RS13960 Protein ID WP_014070883.1
Coordinates 2946214..2946546 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A198GG66
Locus tag ENTAS_RS13965 Protein ID WP_014070884.1
Coordinates 2946547..2946804 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENTAS_RS13940 2941633..2943069 + 1437 WP_041162248.1 anion permease -
ENTAS_RS13945 2943123..2944772 + 1650 WP_014070881.1 class I fumarate hydratase -
ENTAS_RS13950 2945168..2945965 + 798 WP_041162249.1 DgsA anti-repressor MtfA -
ENTAS_RS13960 2946214..2946546 - 333 WP_014070883.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ENTAS_RS13965 2946547..2946804 - 258 WP_014070884.1 antitoxin Antitoxin
ENTAS_RS13970 2947316..2948266 - 951 WP_014070885.1 HTH-type transcriptional regulator Cbl -
ENTAS_RS13975 2948363..2949280 - 918 WP_014070886.1 nitrogen assimilation transcriptional regulator NAC -
ENTAS_RS13985 2949773..2950672 - 900 WP_014070887.1 L,D-transpeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11874.62 Da        Isoelectric Point: 6.9817

>T25635 WP_014070883.1 NC_015968:c2946546-2946214 [Enterobacter soli]
MDRGEIWLVSLDPTSGHEQSGKRPVLIVSPASFNVFTRLPVVVPVTSGGNFAREAGFAVSLDSAVTKTTGVIRCDQPRTI
DMEARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T25635 NC_015968:c2946546-2946214 [Enterobacter soli]
ATGGACCGAGGGGAAATCTGGCTTGTTTCACTGGATCCTACCAGCGGTCACGAACAAAGCGGAAAACGTCCTGTACTCAT
TGTTTCTCCGGCATCATTTAACGTGTTCACCAGGTTACCCGTTGTTGTTCCTGTCACCAGCGGCGGAAACTTTGCCCGCG
AAGCTGGCTTTGCCGTCTCACTGGACAGCGCAGTAACAAAAACAACGGGTGTTATTCGCTGCGATCAACCCAGAACCATT
GATATGGAAGCCCGGAGTGGTAAGCGGCTCGAACGCATACCCGACGCTGTTGTGAATGAAGTGCTGGCCAGACTGGAAGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9249.74 Da        Isoelectric Point: 4.7512

>AT25635 WP_014070884.1 NC_015968:c2946804-2946547 [Enterobacter soli]
MFTTRLKKVGGSVMLAVPPAVLKTLGLSTNSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEDRGWIDAPAV
GKEIL

Download         Length: 258 bp

>AT25635 NC_015968:c2946804-2946547 [Enterobacter soli]
ATGTTTACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTCCCTCCCGCTGTACTGAAAACGCTGGGATT
GTCGACAAACAGCGAAGTGGGCATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGACCTCGTTATTCGC
TCGAAGAACTGCTGGCGCAGTGCGATCCACACGCCGAAATAAGCGAAGAGGATCGGGGGTGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A198GGF1

Antitoxin

Source ID Structure
AlphaFold DB A0A198GG66

References