256289

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4107780..4108356 Replicon chromosome
Accession NZ_CP103710
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag M5S69_RS20315 Protein ID WP_001295743.1
Coordinates 4108069..4108356 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag M5S69_RS20310 Protein ID WP_000063148.1
Coordinates 4107780..4108082 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S69_RS20295 (4104376) 4104376..4106526 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5S69_RS20300 (4106576) 4106576..4106779 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5S69_RS20305 (4106790) 4106790..4107746 + 957 WP_001295745.1 GTPase -
M5S69_RS20310 (4107780) 4107780..4108082 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
M5S69_RS20315 (4108069) 4108069..4108356 - 288 WP_001295743.1 BrnT family toxin Toxin
M5S69_RS20320 (4108553) 4108553..4109719 + 1167 WP_000800831.1 restriction endonuclease -
M5S69_RS20325 (4109783) 4109783..4111402 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
M5S69_RS20330 (4111392) 4111392..4112696 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T256289 WP_001295743.1 NZ_CP103710:c4108356-4108069 [Escherichia coli O25b:H4-ST131]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT256289 WP_000063148.1 NZ_CP103710:c4108082-4107780 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References