256288

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3997794..3998314 Replicon chromosome
Accession NZ_CP103710
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag M5S69_RS19780 Protein ID WP_001160964.1
Coordinates 3997794..3998078 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag M5S69_RS19785 Protein ID WP_000125566.1
Coordinates 3998081..3998314 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S69_RS19755 (3993439) 3993439..3994725 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
M5S69_RS19760 (3994725) 3994725..3995714 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
M5S69_RS19765 (3995711) 3995711..3996532 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
M5S69_RS19770 (3996535) 3996535..3996912 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
M5S69_RS19775 (3996919) 3996919..3997767 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
M5S69_RS19780 (3997794) 3997794..3998078 - 285 WP_001160964.1 CcdB family protein Toxin
M5S69_RS19785 (3998081) 3998081..3998314 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
M5S69_RS19790 (3998400) 3998400..3998879 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
M5S69_RS19795 (3999071) 3999071..4000933 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
M5S69_RS19800 (4000926) 4000926..4001456 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
M5S69_RS19805 (4001564) 4001564..4002895 - 1332 WP_001183209.1 MFS transporter -
M5S69_RS19810 (4002954) 4002954..4003241 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T256288 WP_001160964.1 NZ_CP103710:c3998078-3997794 [Escherichia coli O25b:H4-ST131]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT256288 WP_000125566.1 NZ_CP103710:c3998314-3998081 [Escherichia coli O25b:H4-ST131]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References