256287

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3545957..3546575 Replicon chromosome
Accession NZ_CP103710
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag M5S69_RS17600 Protein ID WP_001291435.1
Coordinates 3546357..3546575 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag M5S69_RS17595 Protein ID WP_000344800.1
Coordinates 3545957..3546331 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S69_RS17585 (3541046) 3541046..3542239 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
M5S69_RS17590 (3542262) 3542262..3545411 + 3150 WP_001132478.1 efflux RND transporter permease AcrB -
M5S69_RS17595 (3545957) 3545957..3546331 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
M5S69_RS17600 (3546357) 3546357..3546575 + 219 WP_001291435.1 HHA domain-containing protein Toxin
M5S69_RS17605 (3546749) 3546749..3547300 + 552 WP_000102539.1 maltose O-acetyltransferase -
M5S69_RS17610 (3547416) 3547416..3547886 + 471 WP_000136192.1 YlaC family protein -
M5S69_RS17615 (3548050) 3548050..3549600 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
M5S69_RS17620 (3549642) 3549642..3549995 - 354 WP_000878135.1 DUF1428 family protein -
M5S69_RS17630 (3550374) 3550374..3550685 + 312 WP_000409908.1 MGMT family protein -
M5S69_RS17635 (3550716) 3550716..3551288 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T256287 WP_001291435.1 NZ_CP103710:3546357-3546575 [Escherichia coli O25b:H4-ST131]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT256287 WP_000344800.1 NZ_CP103710:3545957-3546331 [Escherichia coli O25b:H4-ST131]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References