Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1034042..1034625 Replicon chromosome
Accession NZ_CP103704
Organism Escherichia coli strain ST361

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag M5T41_RS04960 Protein ID WP_061349013.1
Coordinates 1034290..1034625 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag M5T41_RS04955 Protein ID WP_000581937.1
Coordinates 1034042..1034290 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T41_RS04945 (1030381) 1030381..1031682 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
M5T41_RS04950 (1031730) 1031730..1033964 + 2235 WP_000226815.1 GTP pyrophosphokinase -
M5T41_RS04955 (1034042) 1034042..1034290 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
M5T41_RS04960 (1034290) 1034290..1034625 + 336 WP_061349013.1 endoribonuclease MazF Toxin
M5T41_RS04965 (1034696) 1034696..1035487 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
M5T41_RS04970 (1035715) 1035715..1037352 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
M5T41_RS04975 (1037440) 1037440..1038738 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12112.07 Da        Isoelectric Point: 8.2618

>T256252 WP_061349013.1 NZ_CP103704:1034290-1034625 [Escherichia coli]
MVSRYVPDMGDLIWIDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT256252 WP_000581937.1 NZ_CP103704:1034042-1034290 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References