Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5256398..5257023 Replicon chromosome
Accession NZ_CP103699
Organism Klebsiella pneumoniae strain ST307

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M5R30_RS25525 Protein ID WP_002882817.1
Coordinates 5256398..5256781 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M5R30_RS25530 Protein ID WP_004150355.1
Coordinates 5256781..5257023 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R30_RS25510 (5253764) 5253764..5254666 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M5R30_RS25515 (5254663) 5254663..5255298 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M5R30_RS25520 (5255295) 5255295..5256224 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M5R30_RS25525 (5256398) 5256398..5256781 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M5R30_RS25530 (5256781) 5256781..5257023 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M5R30_RS25535 (5257228) 5257228..5258145 + 918 WP_009484979.1 alpha/beta hydrolase -
M5R30_RS25540 (5258159) 5258159..5259100 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M5R30_RS25545 (5259145) 5259145..5259582 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M5R30_RS25550 (5259579) 5259579..5260439 - 861 WP_004146232.1 virulence factor BrkB family protein -
M5R30_RS25555 (5260433) 5260433..5261032 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T256246 WP_002882817.1 NZ_CP103699:c5256781-5256398 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT256246 WP_004150355.1 NZ_CP103699:c5257023-5256781 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References