Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4648576..4649386 Replicon chromosome
Accession NZ_CP103699
Organism Klebsiella pneumoniae strain ST307

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M5R30_RS22685 Protein ID WP_004178461.1
Coordinates 4648576..4649109 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M5R30_RS22690 Protein ID WP_002887278.1
Coordinates 4649120..4649386 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R30_RS22680 (4647407) 4647407..4648528 + 1122 WP_004186850.1 cupin domain-containing protein -
M5R30_RS22685 (4648576) 4648576..4649109 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M5R30_RS22690 (4649120) 4649120..4649386 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M5R30_RS22695 (4649489) 4649489..4650922 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
M5R30_RS22700 (4650912) 4650912..4651595 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
M5R30_RS22705 (4651768) 4651768..4653153 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
M5R30_RS22710 (4653171) 4653171..4653515 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T256242 WP_004178461.1 NZ_CP103699:c4649109-4648576 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT256242 WP_002887278.1 NZ_CP103699:c4649386-4649120 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References