Detailed information of TA system TA025623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 70562..71224
• Replicon: chromosome
• Accession: NC_015968
• Organism: Enterobacter asburiae LF7a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ENTAS_RS00335
• Protein ID: WP_014068311.1
• Coordinates: 70826..71224 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A198GX98
• Locus tag: ENTAS_RS00330
• Protein ID: WP_014068310.1
• Coordinates: 70562..70825 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENTAS_RS00305	65774..66895	-	1122	WP_014068305.1	efflux RND transporter periplasmic adaptor subunit	-
ENTAS_RS00310	67044..67613	-	570	WP_014068306.1	TetR/AcrR family transcriptional regulator	-
ENTAS_RS00315	67800..68219	+	420	WP_014068307.1	GNAT family N-acetyltransferase	-
ENTAS_RS00320	68216..68863	-	648	WP_014068308.1	TetR/AcrR family transcriptional regulator	-
ENTAS_RS00325	68942..70444	+	1503	WP_041161974.1	MFS transporter	-
ENTAS_RS00330	70562..70825	+	264	WP_014068310.1	virulence protein	Antitoxin
ENTAS_RS00335	70826..71224	+	399	WP_014068311.1	type II toxin-antitoxin system VapC family toxin	Toxin
ENTAS_RS00340	71411..72211	+	801	WP_014068312.1	lipoprotein NlpA	-
ENTAS_RS00345	72336..73115	+	780	WP_014068313.1	MBL fold metallo-hydrolase	-
ENTAS_RS00350	73142..74095	+	954	WP_014068314.1	helix-turn-helix domain-containing protein	-
ENTAS_RS00355	74124..75212	-	1089	WP_041161975.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14743.98 Da
• Isoelectric Point: 5.8943

>T25623 WP_014068311.1 NC_015968:70826-71224 [Enterobacter soli]
MRHMLDTNMVSHLVKQHPAVVNQYSRIAPEEMCISSVTEAELLYGIAKKQSITLQETILEFLKTITICHWDSDAAAIYGK
LRADMEKRGKVMGDLDQLIAAHAISRGTTVVTNDHAFAMVQDLTVEDWTTVA

Nucleotide

• Length: 399 bp

>T25623 NC_015968:70826-71224 [Enterobacter soli]
ATGCGGCATATGCTGGATACAAACATGGTGAGCCACCTGGTAAAGCAACATCCTGCGGTGGTCAATCAATATTCCCGAAT
AGCGCCCGAAGAAATGTGTATTTCAAGCGTAACGGAGGCCGAGTTGCTTTATGGCATTGCCAAAAAGCAGAGCATTACAT
TACAAGAAACCATCCTGGAATTTCTTAAAACCATCACCATTTGTCACTGGGACAGTGACGCCGCAGCCATTTACGGCAAA
CTGCGTGCAGACATGGAAAAGCGTGGAAAAGTGATGGGCGATCTCGACCAGCTGATTGCCGCACACGCGATCAGCAGGGG
AACCACGGTGGTCACTAACGATCACGCTTTCGCGATGGTGCAGGATCTTACGGTAGAGGACTGGACTACCGTTGCCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10198.62 Da
• Isoelectric Point: 10.0414

>AT25623 WP_014068310.1 NC_015968:70562-70825 [Enterobacter soli]
MERKAKLFKKGRNQAVVLPAEFAFDTENVYIRRDEQGNVVLTAMSEKARHREIFLRLLKQTHVPDSFLSKEERNQSYTTR
DPFAGLE

Nucleotide

• Length: 264 bp

>AT25623 NC_015968:70562-70825 [Enterobacter soli]
ATGGAACGCAAAGCAAAGCTATTTAAAAAGGGGCGAAATCAGGCCGTTGTTCTGCCTGCGGAATTCGCATTTGATACAGA
GAATGTTTATATCCGTCGGGATGAGCAGGGGAATGTTGTTCTGACGGCAATGTCAGAGAAAGCGCGGCACAGGGAAATCT
TTTTGCGTTTACTAAAACAGACGCATGTGCCTGATTCGTTTCTGAGTAAAGAGGAACGCAATCAAAGCTATACGACAAGA
GATCCTTTTGCAGGGCTAGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A198GX98