Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4085153..4085778 Replicon chromosome
Accession NZ_CP103697
Organism Klebsiella pneumoniae strain ST3576

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag M5R27_RS19700 Protein ID WP_019705794.1
Coordinates 4085153..4085536 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M5R27_RS19705 Protein ID WP_004150355.1
Coordinates 4085536..4085778 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R27_RS19685 (M5R27_19685) 4082519..4083421 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M5R27_RS19690 (M5R27_19690) 4083418..4084053 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M5R27_RS19695 (M5R27_19695) 4084050..4084979 + 930 WP_121915261.1 formate dehydrogenase accessory protein FdhE -
M5R27_RS19700 (M5R27_19700) 4085153..4085536 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
M5R27_RS19705 (M5R27_19705) 4085536..4085778 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M5R27_RS19710 (M5R27_19710) 4085983..4086900 + 918 WP_012737090.1 alpha/beta hydrolase -
M5R27_RS19715 (M5R27_19715) 4086914..4087855 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
M5R27_RS19720 (M5R27_19720) 4087900..4088337 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M5R27_RS19725 (M5R27_19725) 4088334..4089194 - 861 WP_004146232.1 virulence factor BrkB family protein -
M5R27_RS19730 (M5R27_19730) 4089188..4089787 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T256226 WP_019705794.1 NZ_CP103697:c4085536-4085153 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT256226 WP_004150355.1 NZ_CP103697:c4085778-4085536 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References