256216

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 33412..34210 Replicon plasmid pMB3825A_2
Accession NZ_CP103696
Organism Escherichia coli strain ST12468

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag M5T31_RS23650 Protein ID WP_000072677.1
Coordinates 33689..34210 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag M5T31_RS23645 Protein ID WP_001351987.1
Coordinates 33412..33681 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T31_RS23620 (M5T31_23620) 28839..30179 - 1341 WP_000137333.1 DnaB-like helicase C-terminal domain-containing protein -
M5T31_RS23625 (M5T31_23625) 30223..30963 - 741 WP_001717320.1 hypothetical protein -
M5T31_RS23630 (M5T31_23630) 31246..32013 + 768 WP_000342417.1 hypothetical protein -
M5T31_RS23635 (M5T31_23635) 32066..32419 - 354 WP_160378290.1 hypothetical protein -
M5T31_RS23640 (M5T31_23640) 32425..33093 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
M5T31_RS23645 (M5T31_23645) 33412..33681 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
M5T31_RS23650 (M5T31_23650) 33689..34210 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
M5T31_RS23655 (M5T31_23655) 34379..34630 - 252 WP_000901559.1 hypothetical protein -
M5T31_RS23660 (M5T31_23660) 34632..35324 - 693 WP_012640731.1 hypothetical protein -
M5T31_RS23665 (M5T31_23665) 35338..35661 - 324 WP_000064175.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-14 - 1..114012 114012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T256216 WP_000072677.1 NZ_CP103696:33689-34210 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT256216 WP_001351987.1 NZ_CP103696:33412-33681 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References