256212

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21881..22524 Replicon plasmid pMB3825A_1
Accession NZ_CP103695
Organism Escherichia coli strain ST12468

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag M5T31_RS22805 Protein ID WP_001044768.1
Coordinates 22108..22524 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag M5T31_RS22800 Protein ID WP_001261287.1
Coordinates 21881..22111 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T31_RS22780 (17041) 17041..18129 + 1089 WP_000952217.1 transcriptional repressor PifC -
M5T31_RS22785 (18131) 18131..20356 + 2226 WP_000698737.1 P-loop NTPase fold protein -
M5T31_RS22790 (20406) 20406..21305 - 900 WP_000963206.1 nucleotide-binding protein -
M5T31_RS22795 (21295) 21295..21585 - 291 WP_000111771.1 hypothetical protein -
M5T31_RS22800 (21881) 21881..22111 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M5T31_RS22805 (22108) 22108..22524 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
M5T31_RS22810 (22686) 22686..24824 - 2139 WP_000350638.1 AAA family ATPase -
M5T31_RS22815 (25178) 25178..25435 + 258 WP_000343085.1 hypothetical protein -
M5T31_RS22820 (25435) 25435..26025 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IIa / tet(A) / aph(3')-Ia / sitABCD - 1..128470 128470


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T256212 WP_001044768.1 NZ_CP103695:22108-22524 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT256212 WP_001261287.1 NZ_CP103695:21881-22111 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References