Detailed information of TA system TA025621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 147904..148708
• Replicon: plasmid pENTAS01
• Accession: NC_015963
• Organism: Enterobacter asburiae LF7a

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: ENTAS_RS23520
• Protein ID: WP_014063951.1
• Coordinates: 147904..148434 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A198GDR7
• Locus tag: ENTAS_RS23525
• Protein ID: WP_014063952.1
• Coordinates: 148439..148708 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ENTAS_RS23495	143294..144460	+	1167	WP_014063949.1	plasmid-partitioning protein SopA	-
ENTAS_RS23500	144460..145440	+	981	WP_014063950.1	ParB/RepB/Spo0J family plasmid partition protein	-
ENTAS_RS23515	146644..147812	+	1169	WP_088201860.1	IS3 family transposase	-
ENTAS_RS23520	147904..148434	-	531	WP_014063951.1	GNAT family N-acetyltransferase	Toxin
ENTAS_RS23525	148439..148708	-	270	WP_014063952.1	DUF1778 domain-containing protein	Antitoxin
ENTAS_RS23530	149000..149800	-	801	Protein_152	Tn3 family transposase	-
ENTAS_RS23535	149952..151190	-	1239	WP_041162338.1	IS91 family transposase	-
ENTAS_RS23540	151416..152126	-	711	WP_049796706.1	hypothetical protein	-
ENTAS_RS23545	152142..152910	-	769	Protein_155	tyrosine-type recombinase/integrase	-
ENTAS_RS23555	153264..153530	-	267	WP_014063956.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mgtC / mgtB	1..166725	166725
	-	inside	IScluster/Tn	-	-	146644..149803	3159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 19807.62 Da
• Isoelectric Point: 6.8639

>T25621 WP_014063951.1 NC_015963:c148434-147904 [Enterobacter soli]
VDGLKVEIFSEEVEYDLSNFDCGEEYLNTFLTDHLKRQHIGKVLRGYVLVTREDKPRVMGYYTLSGSCFEKEQLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIQRQGYGDALVTHAMKVVYLASQAVGIHGMFVEALNDNAKKFYLGLGFVQLKGANSNAL
FYPTKAIEELFGAQDE

Nucleotide

• Length: 531 bp

>T25621 NC_015963:c148434-147904 [Enterobacter soli]
GTGGACGGTTTAAAGGTAGAGATTTTTTCTGAGGAAGTTGAATATGATTTAAGCAACTTCGATTGTGGCGAAGAGTACCT
TAATACTTTTCTCACTGACCACCTGAAACGCCAACACATCGGCAAAGTATTACGAGGTTATGTGCTCGTCACGAGAGAAG
ATAAGCCTCGTGTTATGGGGTACTACACGTTGTCCGGGAGCTGCTTCGAAAAAGAGCAGCTTCCAAGCAAAACGCAGCAA
AAACGAGTGCCCTATAAAAACGTACCGAGTGTTACTCTGGGGCGTTTGGCAATTGATAAAAGTATTCAACGCCAGGGTTA
TGGAGACGCGCTTGTAACACATGCCATGAAGGTTGTGTATCTTGCATCTCAAGCGGTAGGAATACATGGAATGTTCGTTG
AAGCGCTTAATGACAACGCCAAAAAATTCTACCTTGGATTAGGCTTCGTTCAGCTCAAGGGTGCGAACAGCAACGCCCTC
TTCTATCCAACCAAAGCGATAGAAGAGCTATTCGGAGCTCAAGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10192.65 Da
• Isoelectric Point: 7.0167

>AT25621 WP_014063952.1 NC_015963:c148708-148439 [Enterobacter soli]
MSALKKQRIDLRLNEDDKSMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLILNEESWNLVMEAISNPPAPNDRLKR
AAKRLQKLE

Nucleotide

• Length: 270 bp

>AT25621 NC_015963:c148708-148439 [Enterobacter soli]
ATGTCAGCACTGAAGAAACAGCGCATCGATCTTAGATTAAACGAAGACGACAAAAGCATGATCGAAGAAGCCGCGGCTAT
GACTAACCAGTCAATTTCACAGTTTATGGTTAGCACAGCCTCTGAACGTGCAGCGGAAGTTATTGATCAGCACCGCCGCC
TGATTCTTAACGAGGAATCATGGAACCTGGTAATGGAAGCGATCAGTAACCCACCGGCACCGAACGATCGACTGAAACGA
GCTGCTAAGCGTCTCCAGAAATTGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A198GDR7