Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2102544..2103172 Replicon chromosome
Accession NC_015958
Organism Thermoanaerobacter wiegelii Rt8.B1

Toxin (Protein)


Gene name mazF Uniprot ID B0KD57
Locus tag THEWI_RS10280 Protein ID WP_003867472.1
Coordinates 2102544..2102894 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M8DD10
Locus tag THEWI_RS10285 Protein ID WP_003870023.1
Coordinates 2102897..2103172 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
THEWI_RS10260 2098116..2099207 - 1092 WP_014063368.1 polysaccharide pyruvyl transferase CsaB -
THEWI_RS10265 2099209..2101221 - 2013 WP_014063369.1 hypothetical protein -
THEWI_RS10270 2101336..2101788 - 453 WP_003870021.1 hypothetical protein -
THEWI_RS14275 2101932..2102090 - 159 WP_014062067.1 hypothetical protein -
THEWI_RS10280 2102544..2102894 - 351 WP_003867472.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
THEWI_RS10285 2102897..2103172 - 276 WP_003870023.1 ribbon-helix-helix protein, CopG family Antitoxin
THEWI_RS10290 2103274..2104428 - 1155 WP_014063370.1 alanine racemase -
THEWI_RS10295 2104448..2105458 - 1011 WP_014063371.1 outer membrane lipoprotein carrier protein LolA -
THEWI_RS10300 2105470..2107002 - 1533 WP_014063372.1 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase -
THEWI_RS10305 2106965..2107381 - 417 WP_003870027.1 holo-ACP synthase -
THEWI_RS10310 2107477..2107842 - 366 WP_003867466.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12933.02 Da        Isoelectric Point: 6.4714

>T25620 WP_003867472.1 NC_015958:c2102894-2102544 [Thermoanaerobacter wiegelii Rt8.B1]
MVIKRGDIFYADLSPVIGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHVEINGAEYGLNKDSVVLLEQI
RTIDKKRLREKIGHFDQEMMEKVNEALQISLGLIDF

Download         Length: 351 bp

>T25620 NC_015958:c2102894-2102544 [Thermoanaerobacter wiegelii Rt8.B1]
ATGGTGATAAAGAGAGGAGATATCTTTTATGCCGATTTAAGCCCTGTAATAGGCTCTGAACAGGGTGGAATTCGGCCTGT
ACTTATAATTCAAAATGATATTGGTAATAAATATAGTCCAACAGTAATAGTAGCGGCAATTACATCTCAGATCAACAAAG
CTAAATTGCCCACTCATGTTGAAATAAATGGGGCAGAATATGGACTTAACAAAGATTCTGTAGTATTGTTAGAACAAATC
AGGACTATTGACAAAAAACGTTTAAGAGAAAAAATTGGCCATTTTGACCAGGAAATGATGGAGAAAGTCAATGAAGCTTT
GCAAATAAGTTTAGGGTTGATTGATTTTTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10547.32 Da        Isoelectric Point: 6.3662

>AT25620 WP_003870023.1 NC_015958:c2103172-2102897 [Thermoanaerobacter wiegelii Rt8.B1]
VGETKRILVSLPQSLLEEVDVLAAMENRNRSEFIREAMKLYIRERKKVQIRESMKKGYLEMAAINSELAEMGLTAENECF
TGYEMKLKKCD

Download         Length: 276 bp

>AT25620 NC_015958:c2103172-2102897 [Thermoanaerobacter wiegelii Rt8.B1]
GTGGGCGAAACAAAGCGAATACTTGTGAGCTTACCTCAGAGTTTATTAGAAGAGGTCGACGTTCTTGCCGCTATGGAAAA
CAGAAATCGCAGTGAATTTATAAGAGAAGCGATGAAATTATATATACGCGAGAGGAAAAAAGTTCAAATACGCGAGAGCA
TGAAAAAAGGATATCTCGAGATGGCAGCAATCAACAGTGAACTTGCGGAAATGGGCCTAACCGCAGAAAACGAATGTTTT
ACAGGATATGAAATGAAATTGAAAAAGTGTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B3BRL3

Antitoxin

Source ID Structure
AlphaFold DB M8DD10

References