Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53667..54403 Replicon plasmid pMB3935_1
Accession NZ_CP103688
Organism Klebsiella pneumoniae strain ST307

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag M5S48_RS26400 Protein ID WP_003026803.1
Coordinates 53921..54403 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag M5S48_RS26395 Protein ID WP_003026799.1
Coordinates 53667..53933 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S48_RS26350 (M5S48_26350) 49729..50091 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
M5S48_RS26355 (M5S48_26355) 50141..50491 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
M5S48_RS26360 (M5S48_26360) 50849..51118 + 270 WP_004152102.1 hypothetical protein -
M5S48_RS26365 (M5S48_26365) 51106..51681 + 576 WP_004152103.1 hypothetical protein -
M5S48_RS26370 (M5S48_26370) 51712..52206 + 495 WP_004152104.1 DNA-binding protein -
M5S48_RS26375 (M5S48_26375) 52250..52618 + 369 WP_004152105.1 hypothetical protein -
M5S48_RS26380 (M5S48_26380) 52652..52855 + 204 WP_004152106.1 HHA domain-containing protein -
M5S48_RS26385 (M5S48_26385) 52904..53161 + 258 WP_004152107.1 hypothetical protein -
M5S48_RS26390 (M5S48_26390) 53237..53491 + 255 WP_004152108.1 hypothetical protein -
M5S48_RS26395 (M5S48_26395) 53667..53933 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
M5S48_RS26400 (M5S48_26400) 53921..54403 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
M5S48_RS26405 (M5S48_26405) 54615..55961 + 1347 WP_020314316.1 ISNCY family transposase -
M5S48_RS26410 (M5S48_26410) 56122..56253 + 132 WP_004218042.1 hypothetical protein -
M5S48_RS26415 (M5S48_26415) 56462..57627 - 1166 Protein_59 IS3 family transposase -
M5S48_RS26420 (M5S48_26420) 57804..58766 - 963 WP_004152113.1 zinc metalloprotease HtpX -
M5S48_RS26425 (M5S48_26425) 58753..59241 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IIa / blaTEM-1B / blaCTX-M-15 - 1..183966 183966


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T256194 WP_003026803.1 NZ_CP103688:53921-54403 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT256194 WP_003026799.1 NZ_CP103688:53667-53933 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References