Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 253271..253797 Replicon plasmid pMB4528V_1
Accession NZ_CP103684
Organism Enterobacter hormaechei strain ST90

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag M5S84_RS24135 Protein ID WP_000323025.1
Coordinates 253271..253558 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag M5S84_RS24140 Protein ID WP_000534858.1
Coordinates 253558..253797 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S84_RS24075 (M5S84_24075) 248606..248989 + 384 WP_050596975.1 hypothetical protein -
M5S84_RS24080 (M5S84_24080) 249000..249254 + 255 WP_032610479.1 hypothetical protein -
M5S84_RS24085 (M5S84_24085) 249254..249670 + 417 WP_032610480.1 hypothetical protein -
M5S84_RS24090 (M5S84_24090) 249670..249801 + 132 WP_255344485.1 hypothetical protein -
M5S84_RS24095 (M5S84_24095) 249788..250069 + 282 WP_032610524.1 hypothetical protein -
M5S84_RS24100 (M5S84_24100) 250255..250809 + 555 WP_015063016.1 hypothetical protein -
M5S84_RS24105 (M5S84_24105) 250869..251117 + 249 WP_050596976.1 hypothetical protein -
M5S84_RS24110 (M5S84_24110) 251242..251574 + 333 WP_032610481.1 DUF5417 domain-containing protein -
M5S84_RS24115 (M5S84_24115) 251619..252188 + 570 WP_032610483.1 hypothetical protein -
M5S84_RS24120 (M5S84_24120) 252212..252562 + 351 WP_032610485.1 hypothetical protein -
M5S84_RS24125 (M5S84_24125) 252602..252808 + 207 WP_044255297.1 hypothetical protein -
M5S84_RS24130 (M5S84_24130) 253042..253200 - 159 WP_064758473.1 type I toxin-antitoxin system Hok family toxin -
M5S84_RS24135 (M5S84_24135) 253271..253558 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
M5S84_RS24140 (M5S84_24140) 253558..253797 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
M5S84_RS24145 (M5S84_24145) 253822..253926 + 105 Protein_279 protein YdfV -
M5S84_RS24150 (M5S84_24150) 254060..254983 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
M5S84_RS24155 (M5S84_24155) 255183..255755 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
M5S84_RS24160 (M5S84_24160) 256231..257469 - 1239 WP_259409756.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSHV-12 / ant(2'')-Ia / catB3 / qacE / sul1 / qnrB17 vipA/tssB / vipB/tssC 1..398127 398127


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T256176 WP_000323025.1 NZ_CP103684:c253558-253271 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT256176 WP_000534858.1 NZ_CP103684:c253797-253558 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References