Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5239049..5239674 Replicon chromosome
Accession NZ_CP103676
Organism Klebsiella pneumoniae strain 2946

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M5S99_RS25360 Protein ID WP_002882817.1
Coordinates 5239049..5239432 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M5S99_RS25365 Protein ID WP_004150355.1
Coordinates 5239432..5239674 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S99_RS25345 (5236415) 5236415..5237317 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M5S99_RS25350 (5237314) 5237314..5237949 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M5S99_RS25355 (5237946) 5237946..5238875 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M5S99_RS25360 (5239049) 5239049..5239432 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M5S99_RS25365 (5239432) 5239432..5239674 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M5S99_RS25370 (5239879) 5239879..5240796 + 918 WP_009484979.1 alpha/beta hydrolase -
M5S99_RS25375 (5240810) 5240810..5241751 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M5S99_RS25380 (5241796) 5241796..5242233 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M5S99_RS25385 (5242230) 5242230..5243090 - 861 WP_004146232.1 virulence factor BrkB family protein -
M5S99_RS25390 (5243084) 5243084..5243683 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T256141 WP_002882817.1 NZ_CP103676:c5239432-5239049 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT256141 WP_004150355.1 NZ_CP103676:c5239674-5239432 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References