Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4586575..4587072 Replicon chromosome
Accession NZ_CP103673
Organism Escherichia coli strain 4069

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M4U10_RS21915 Protein ID WP_001173343.1
Coordinates 4586575..4586748 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M4U10_RS21920 Protein ID WP_000398619.1
Coordinates 4586776..4587072 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4U10_RS21905 (4583245) 4583245..4584702 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
M4U10_RS21910 (4584939) 4584939..4586456 + 1518 WP_001550472.1 lysine--tRNA ligase -
M4U10_RS21915 (4586575) 4586575..4586748 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M4U10_RS21920 (4586776) 4586776..4587072 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M4U10_RS21925 (4587301) 4587301..4587573 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M4U10_RS21930 (4587585) 4587585..4587815 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
M4U10_RS21935 (4587995) 4587995..4589626 + 1632 WP_001216477.1 sensor histidine kinase -
M4U10_RS21940 (4589623) 4589623..4590342 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T256113 WP_001173343.1 NZ_CP103673:c4586748-4586575 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT256113 WP_000398619.1 NZ_CP103673:c4587072-4586776 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References