Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1991089..1991873 Replicon chromosome
Accession NZ_CP103673
Organism Escherichia coli strain 4069

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag M4U10_RS09355 Protein ID WP_000613626.1
Coordinates 1991089..1991583 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag M4U10_RS09360 Protein ID WP_001110447.1
Coordinates 1991580..1991873 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4U10_RS09335 (1986282) 1986282..1987379 + 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -
M4U10_RS09340 (1987379) 1987379..1988320 + 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
M4U10_RS09345 (1988386) 1988386..1990029 + 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
M4U10_RS09350 (1990041) 1990041..1990994 + 954 WP_001212768.1 flagellar hook-associated protein FlgL -
M4U10_RS09355 (1991089) 1991089..1991583 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
M4U10_RS09360 (1991580) 1991580..1991873 - 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
M4U10_RS09365 (1992006) 1992006..1995191 - 3186 WP_001550951.1 ribonuclease E -
M4U10_RS09370 (1995764) 1995764..1996723 + 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T256100 WP_000613626.1 NZ_CP103673:c1991583-1991089 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT256100 WP_001110447.1 NZ_CP103673:c1991873-1991580 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References