256098

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 1767255..1768975 Replicon chromosome
Accession NZ_CP103673
Organism Escherichia coli strain 4069

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag M4U10_RS08300 Protein ID WP_000312833.1
Coordinates 1768319..1768975 (-) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag M4U10_RS08295 Protein ID WP_000634203.1
Coordinates 1767255..1768322 (-) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4U10_RS08280 (1762466) 1762466..1763080 - 615 Protein_1613 IS3 family transposase -
M4U10_RS08285 (1763425) 1763425..1764141 - 717 WP_001122107.1 SprT family zinc-dependent metalloprotease -
M4U10_RS08290 (1764141) 1764141..1767236 - 3096 WP_000108735.1 HsdR family type I site-specific deoxyribonuclease -
M4U10_RS08295 (1767255) 1767255..1768322 - 1068 WP_000634203.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
M4U10_RS08300 (1768319) 1768319..1768975 - 657 WP_000312833.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
M4U10_RS08305 (1768988) 1768988..1770322 - 1335 WP_000228013.1 restriction endonuclease subunit S -
M4U10_RS08310 (1770322) 1770322..1772760 - 2439 WP_000985465.1 type I restriction-modification system subunit M -
M4U10_RS08315 (1772800) 1772800..1773039 - 240 WP_001431807.1 hypothetical protein -
M4U10_RS08320 (1773321) 1773321..1773575 + 255 WP_154670580.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - iroN / iroE / iroE / iroD / iroC / aggD / afaC-I 1741698..1786289 44591


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T256098 WP_000312833.1 NZ_CP103673:c1768975-1768319 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT256098 WP_000634203.1 NZ_CP103673:c1768322-1767255 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References