Detailed information of TA system TA025609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-vapB/VagC(antitoxin)
• Location: 2914669..2915539
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: G0JTS8
• Locus tag: ACIFE_RS14265
• Protein ID: WP_014030285.1
• Coordinates: 2915033..2915539 (-)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0JTS7
• Locus tag: ACIFE_RS14260
• Protein ID: WP_014030284.1
• Coordinates: 2914669..2914899 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS14215	2909693..2910370	-	678	WP_014030274.1	hypothetical protein	-
ACIFE_RS14220	2910391..2910687	-	297	WP_014030275.1	type II toxin-antitoxin system PemK/MazF family toxin	-
ACIFE_RS14225	2910681..2910917	-	237	WP_014030276.1	AbrB family transcriptional regulator	-
ACIFE_RS14230	2911455..2912036	-	582	WP_041651225.1	SocA family protein	-
ACIFE_RS14235	2912111..2912761	-	651	WP_014030279.1	hypothetical protein	-
ACIFE_RS14240	2912767..2913222	-	456	WP_014030280.1	DUF29 domain-containing protein	-
ACIFE_RS14245	2913236..2913457	-	222	WP_014030281.1	hypothetical protein	-
ACIFE_RS14250	2913478..2914128	-	651	WP_014030282.1	hypothetical protein	-
ACIFE_RS14255	2914259..2914672	-	414	WP_014030283.1	tRNA(fMet)-specific endonuclease VapC	-
ACIFE_RS14260	2914669..2914899	-	231	WP_014030284.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ACIFE_RS14265	2915033..2915539	-	507	WP_014030285.1	GNAT family N-acetyltransferase	Toxin
ACIFE_RS14270	2915536..2915814	-	279	WP_014030286.1	DUF1778 domain-containing protein	-
ACIFE_RS14275	2915918..2919280	-	3363	WP_014030287.1	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2863364..2936661	73297

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-147)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18454.45 Da
• Isoelectric Point: 8.8020

>T25609 WP_014030285.1 NC_015942:c2915539-2915033 [Acidithiobacillus ferrivorans SS3]
VSVAYEPVRKLTPTDLVDGFDCGQPALNQFLQRYALVNQKANSAQTYVCCQEKVVVGFYSLAAGSVDPESAPPRVMKGLA
RHPVPVLILARLAVDRNHQGKGLGKTLLKDALLRTVQAADIAGIRCLLVHAKDKAARQWYEAWDFEPSPTDPYHLFLMLK
DLKALLAG

Nucleotide

• Length: 507 bp

>T25609 NC_015942:c2915539-2915033 [Acidithiobacillus ferrivorans SS3]
GTGAGTGTAGCCTATGAACCGGTCCGAAAGCTCACCCCTACGGATCTGGTCGATGGGTTTGACTGTGGCCAACCAGCACT
GAACCAGTTCCTGCAGCGTTACGCTCTCGTCAATCAGAAGGCCAACAGCGCACAAACCTACGTTTGCTGCCAGGAAAAAG
TCGTAGTTGGGTTCTACAGCCTCGCAGCCGGCAGCGTCGATCCCGAGTCGGCCCCGCCGAGGGTGATGAAGGGCCTGGCC
AGACACCCGGTGCCCGTGTTGATCTTGGCCAGACTCGCCGTTGATCGAAATCACCAGGGCAAAGGCCTTGGCAAAACGCT
CCTCAAGGACGCCCTGCTGCGCACTGTGCAGGCGGCCGATATTGCCGGCATACGTTGCCTGCTGGTCCATGCCAAGGACA
AGGCAGCCCGGCAATGGTATGAGGCCTGGGACTTCGAGCCAAGCCCGACCGATCCATACCACCTTTTCCTGATGCTCAAG
GATCTCAAAGCGTTACTAGCGGGGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8559.74 Da
• Isoelectric Point: 4.7063

>AT25609 WP_014030284.1 NC_015942:c2914899-2914669 [Acidithiobacillus ferrivorans SS3]
MEQATVFQINRSQAVRLPKSIAFPADVKRVDVVAMGRVRILIPAGESWESWFDGEGVSADFMESREQPANQIREPL

Nucleotide

• Length: 231 bp

>AT25609 NC_015942:c2914899-2914669 [Acidithiobacillus ferrivorans SS3]
ATGGAACAAGCCACCGTTTTTCAGATCAACCGTAGCCAGGCGGTACGCCTACCCAAGTCGATAGCCTTCCCGGCAGACGT
GAAGCGCGTGGATGTGGTCGCCATGGGCCGTGTGCGTATTCTCATTCCTGCCGGAGAGTCCTGGGAGAGCTGGTTTGATG
GAGAAGGGGTCAGCGCCGACTTCATGGAGAGTCGAGAGCAACCCGCCAACCAGATCCGGGAACCGCTGTGA

Structures

Toxin

• AlphaFold DB: G0JTS8

Antitoxin

• AlphaFold DB: G0JTS7