Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4454814..4455390 Replicon chromosome
Accession NZ_CP103672
Organism Escherichia coli O25b:H4-ST131 strain 4433

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag M5S92_RS22290 Protein ID WP_001295743.1
Coordinates 4455103..4455390 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag M5S92_RS22285 Protein ID WP_000063148.1
Coordinates 4454814..4455116 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S92_RS22270 (4451410) 4451410..4453560 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5S92_RS22275 (4453610) 4453610..4453813 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5S92_RS22280 (4453824) 4453824..4454780 + 957 WP_001295745.1 GTPase -
M5S92_RS22285 (4454814) 4454814..4455116 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
M5S92_RS22290 (4455103) 4455103..4455390 - 288 WP_001295743.1 BrnT family toxin Toxin
M5S92_RS22295 (4455587) 4455587..4456753 + 1167 WP_000800831.1 restriction endonuclease -
M5S92_RS22300 (4456817) 4456817..4458436 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
M5S92_RS22305 (4458426) 4458426..4459730 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T256086 WP_001295743.1 NZ_CP103672:c4455390-4455103 [Escherichia coli O25b:H4-ST131]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT256086 WP_000063148.1 NZ_CP103672:c4455116-4454814 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References