256069

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 25666..26159 Replicon plasmid pMB5646_5
Accession NZ_CP103662
Organism Escherichia coli strain 3150

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag M5R25_RS27215 Protein ID WP_001230709.1
Coordinates 25666..25920 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag M5R25_RS27220 Protein ID WP_000151456.1
Coordinates 25917..26159 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R25_RS27190 (M5R25_27190) 20954..22216 - 1263 WP_000608644.1 IS1380-like element ISEcp1 family transposase -
M5R25_RS27195 (M5R25_27195) 22993..23337 + 345 WP_001146936.1 type II toxin-antitoxin system RelE/ParE family toxin -
M5R25_RS27200 (M5R25_27200) 23324..23674 + 351 WP_000127482.1 addiction module antitoxin -
M5R25_RS27205 (M5R25_27205) 24508..25023 + 516 WP_001025393.1 J domain-containing protein -
M5R25_RS27210 (M5R25_27210) 25127..25669 + 543 WP_001083904.1 hypothetical protein -
M5R25_RS27215 (M5R25_27215) 25666..25920 + 255 WP_001230709.1 hypothetical protein Toxin
M5R25_RS27220 (M5R25_27220) 25917..26159 + 243 WP_000151456.1 hypothetical protein Antitoxin
M5R25_RS27225 (M5R25_27225) 26706..26999 + 294 WP_112367531.1 hypothetical protein -
M5R25_RS27230 (M5R25_27230) 27307..27594 + 288 WP_000606467.1 hypothetical protein -
M5R25_RS27235 (M5R25_27235) 28022..28255 + 234 WP_000093745.1 hypothetical protein -
M5R25_RS27240 (M5R25_27240) 28331..28633 + 303 WP_000379301.1 hypothetical protein -
M5R25_RS27245 (M5R25_27245) 28905..29054 + 150 WP_000903193.1 hypothetical protein -
M5R25_RS27250 (M5R25_27250) 29165..29986 + 822 WP_073972859.1 DUF932 domain-containing protein -
M5R25_RS27255 (M5R25_27255) 30282..30872 - 591 WP_000252684.1 transglycosylase SLT domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 - 1..51731 51731
- flank IS/Tn blaCTX-M-15 - 19823..22216 2393


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T256069 WP_001230709.1 NZ_CP103662:25666-25920 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT256069 WP_000151456.1 NZ_CP103662:25917-26159 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References