Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 33507..34305 Replicon plasmid pMB5646_2
Accession NZ_CP103659
Organism Escherichia coli strain 3150

Toxin (Protein)


Gene name ataT Uniprot ID A0A0K4UCL9
Locus tag M5R25_RS25675 Protein ID WP_024173399.1
Coordinates 33784..34305 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag M5R25_RS25670 Protein ID WP_001351987.1
Coordinates 33507..33776 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R25_RS25645 (M5R25_25645) 28937..30274 - 1338 WP_228598670.1 DnaB-like helicase C-terminal domain-containing protein -
M5R25_RS25650 (M5R25_25650) 30318..31058 - 741 WP_053271988.1 hypothetical protein -
M5R25_RS25655 (M5R25_25655) 31341..32108 + 768 WP_000342417.1 hypothetical protein -
M5R25_RS25660 (M5R25_25660) 32161..32514 - 354 WP_160378290.1 hypothetical protein -
M5R25_RS25665 (M5R25_25665) 32520..33188 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
M5R25_RS25670 (M5R25_25670) 33507..33776 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
M5R25_RS25675 (M5R25_25675) 33784..34305 + 522 WP_024173399.1 GNAT family N-acetyltransferase Toxin
M5R25_RS25680 (M5R25_25680) 34473..34724 - 252 WP_001404395.1 hypothetical protein -
M5R25_RS25685 (M5R25_25685) 34726..35418 - 693 WP_000856757.1 hypothetical protein -
M5R25_RS25690 (M5R25_25690) 35432..35755 - 324 WP_000064175.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..109579 109579


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19475.31 Da        Isoelectric Point: 8.6344

>T256067 WP_024173399.1 NZ_CP103659:33784-34305 [Escherichia coli]
VDNIKIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEGRPKVLGYYTLSGSCFEKESLPSRSQQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLDFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT256067 WP_001351987.1 NZ_CP103659:33507-33776 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4UCL9

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References