Detailed information of TA system TA025606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2910391..2910917
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ACIFE_RS14220
• Protein ID: WP_014030275.1
• Coordinates: 2910391..2910687 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: G0JTR9
• Locus tag: ACIFE_RS14225
• Protein ID: WP_014030276.1
• Coordinates: 2910681..2910917 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS14190	2906548..2906781	+	234	WP_041650023.1	hypothetical protein	-
ACIFE_RS14195	2906866..2907453	-	588	WP_014030271.1	hypothetical protein	-
ACIFE_RS14200	2907864..2908832	+	969	WP_014030272.1	ParM/StbA family protein	-
ACIFE_RS14205	2908855..2909334	+	480	WP_014030273.1	hypothetical protein	-
ACIFE_RS14210	2909338..2909664	+	327	WP_009569263.1	YbjQ family protein	-
ACIFE_RS14215	2909693..2910370	-	678	WP_014030274.1	hypothetical protein	-
ACIFE_RS14220	2910391..2910687	-	297	WP_014030275.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ACIFE_RS14225	2910681..2910917	-	237	WP_014030276.1	AbrB family transcriptional regulator	Antitoxin
ACIFE_RS14230	2911455..2912036	-	582	WP_041651225.1	SocA family protein	-
ACIFE_RS14235	2912111..2912761	-	651	WP_014030279.1	hypothetical protein	-
ACIFE_RS14240	2912767..2913222	-	456	WP_014030280.1	DUF29 domain-containing protein	-
ACIFE_RS14245	2913236..2913457	-	222	WP_014030281.1	hypothetical protein	-
ACIFE_RS14250	2913478..2914128	-	651	WP_014030282.1	hypothetical protein	-
ACIFE_RS14255	2914259..2914672	-	414	WP_014030283.1	tRNA(fMet)-specific endonuclease VapC	-
ACIFE_RS14260	2914669..2914899	-	231	WP_014030284.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
ACIFE_RS14265	2915033..2915539	-	507	WP_014030285.1	GNAT family N-acetyltransferase	-
ACIFE_RS14270	2915536..2915814	-	279	WP_014030286.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2863364..2936661	73297

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10843.77 Da
• Isoelectric Point: 10.6545

>T25606 WP_014030275.1 NC_015942:c2910687-2910391 [Acidithiobacillus ferrivorans SS3]
VVKRGEVYWVNVGTEIRKTRPALIVSPDDLNAALPRVIIALLTSGGQRLGCRPELEFAGKSARILLDQIRTVDKRRLSGK
IGEIPITAWHSVLIEMLS

Nucleotide

• Length: 297 bp

>T25606 NC_015942:c2910687-2910391 [Acidithiobacillus ferrivorans SS3]
GTGGTGAAGCGTGGCGAGGTGTATTGGGTGAATGTCGGCACGGAAATCAGAAAGACGCGCCCGGCGCTGATTGTCTCGCC
AGACGATCTAAACGCCGCTCTGCCCCGTGTCATCATTGCTCTCCTCACCAGCGGTGGACAACGGCTAGGTTGCCGCCCCG
AGTTGGAATTTGCGGGTAAGTCTGCACGCATCCTGCTCGATCAGATCCGTACTGTAGACAAACGCCGTCTATCAGGGAAA
ATAGGCGAGATACCGATTACAGCATGGCATTCTGTGCTTATAGAAATGCTGAGTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8751.86 Da
• Isoelectric Point: 4.1953

>AT25606 WP_014030276.1 NC_015942:c2910917-2910681 [Acidithiobacillus ferrivorans SS3]
MRTSIRKIGNSRGILIPSAFLTDCGIGDHVDILMEGKSLLIRPVQEPRAGWFEGYHSEIDVEPLGDLPVDEGDEDWTW

Nucleotide

• Length: 237 bp

>AT25606 NC_015942:c2910917-2910681 [Acidithiobacillus ferrivorans SS3]
ATGCGTACCAGCATACGGAAAATTGGTAATTCACGCGGCATTCTCATTCCATCTGCGTTTCTGACGGACTGTGGAATCGG
CGACCATGTAGACATTCTCATGGAGGGTAAGTCTCTGCTCATAAGGCCGGTACAAGAGCCGCGTGCCGGATGGTTTGAAG
GTTACCATTCGGAAATTGACGTGGAGCCCTTGGGCGACTTGCCCGTTGATGAAGGCGACGAGGACTGGACGTGGTGA

Structures

Antitoxin

• AlphaFold DB: G0JTR9